Publications by authors named "Sarah Isler"
Article Synopsis
- The R-loop is an RNA-DNA hybrid structure that can form during gene transcription and has implications for health and disease, but mechanisms for its removal are not fully understood.
- Researchers found that the Smc5/6 complex, which is important for chromosome structure, is vital for eliminating these R-loops.
- In experiments with yeast mutants lacking the Smc5/6 complex, there was a significant buildup of R-loops, highlighting the complex's role in targeting specific R-loop structures to promote their degradation with the RNase H2 enzyme.
Objective: To compare behavioural and electrophysiological variables of mice undergoing gas euthanasia with either xenon (Xe) or carbon dioxide (CO).
Study Design: Single animals chronically instrumented for electroencephalography (EEG) recording were randomized to undergo euthanasia with either CO or Xe (n = 6 animals per group).
Animals: Twelve adult (>6 weeks old) male C57Bl6/n mice.
Carbon dioxide (CO2) is one of the most commonly used gas euthanasia agents in mice, despite reports of aversion and nociception. Inert gases such as nitrogen (N2) may be a viable alternative to carbon dioxide. Here we compared behavioural and electrophysiological reactions to CO2 or N2 at either slow fill or rapid fill in C57Bl/6 mice undergoing gas euthanasia.
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