Publications by authors named "Sarah Elizabeth Bohndiek"
Understanding the impact of free radicals and antioxidants in cell biology is vital; however, noninvasive nonperturbative imaging of oxidative stress remains a challenge. Here, we evaluated the ability of label-free Raman spectroscopy to monitor redox biochemical changes in antioxidant (-acetyl-l-cysteine, NAC) and pro-oxidant (tert-butyl hydroperoxide, TBHP) environments. Cellular changes were compared to fluorescence microscopy using CellROX Orange as a marker of oxidative stress.
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- - Traumatic brain injury (TBI) involves a complex interaction between the initial injury, secondary damage, and the body's response, making it a varied and complicated condition that warrants targeted interventions.
- - Current therapies based on biological understanding have not successfully transitioned into effective clinical treatments, partly due to the limitations in accurately characterizing the biological aspects of TBI.
- - The review discusses advanced technologies, like cerebral microdialysis and multiplex proteomic techniques, that can enhance the understanding of TBI by allowing for detailed biochemical analysis, while also addressing the challenges in managing the resulting large-scale data.
Traumatic brain injury (TBI) constitutes a major cause of death and long-term disability. At present, we lack methods to non-invasively track tissue biochemistry and hence select appropriate interventions for patients. We hypothesized that detailed label-free vibrational chemical analysis of focal TBI could provide such information.
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