Treatment outcome in children with nontuberculous mycobacterial lymphadenitis: A retrospective follow-up study.

Introduction: Information on the long-term treatment outcome following nontuberculous mycobacterial (NTM) lymphadenitis is very limited. We performed a study to (a) compare cure rates following different initial treatment courses, (b) describe subsequent treatment courses and their outcomes, and (c) determine the occurrence of late sequelae in immunocompetent children with NTM lymphadenitis.

Materials And Methods: In 2011, we conducted a retrospective follow-up study in 71 parents whose children had been hospitalized with NTM disease 2002-2005.

Estrogenicity of novel phase I and phase II metabolites of zearalenone and cis-zearalenone.

Zearalenone and its cis-isomer, cis-zearalenone, are nonsteroidal mycotoxins that elicit an estrogenic response upon binding to the estrogen receptor. This study compares the estrogenicity of eleven congeners including novel metabolites as 15-OH-zearalenone, zearalenone-14-sulfate, α-cis-zearalenol and β-cis-zearalenol using the E-Screen assay. Overall, a change in the configuration from trans to cis retains significant estrogenic activity.

Automated solid-phase extraction coupled online with HPLC-FLD for the quantification of zearalenone in edible oil.

Established maximum levels for the mycotoxin zearalenone (ZEN) in edible oil require monitoring by reliable analytical methods. Therefore, an automated SPE-HPLC online system based on dynamic covalent hydrazine chemistry has been developed. The SPE step comprises a reversible hydrazone formation by ZEN and a hydrazine moiety covalently attached to a solid phase.

In vitro phase I metabolism of cis-zearalenone.

The present study investigates the in vitro phase I metabolism of cis-zearalenone (cis-ZEN) in rat liver microsomes and human liver microsomes. cis-ZEN is an often ignored isomer of the trans-configured Fusarium mycotoxin zearalenone (trans-ZEN). Upon the influence of (UV-) light, trans-ZEN isomerizes to cis-ZEN.

(3S,7R)-7,14,16-Trihy-droxy-3-methyl-3,4,5,6,7,8,9,10,11,12-deca-hydro-1H-2-benzoxacyclo-tetra-decin-1-one.

The asymmetric unit of the title compound, C(18)H(26)O(5), which is known as α-zearalanol, contains two mol-ecules having the same conformation, with a r.m.s.

Photochemical trans-/cis-isomerization and quantitation of zearalenone in edible oils.

The emphasis of the present work was to investigate the photochemical conversion of trans- to cis-zearalenone in edible oils under real-life conditions. For quantitation purposes a cis-zearalenone standard was synthesized and characterized for its identity and purity (≥95%) by (1)H NMR, X-ray crystallography, HPLC fluorescence and mass spectrometric detection. In a sample survey of 12 edible oils (9 corn oils, 3 hempseed oils) from local supermarkets all corn oils contained trans-zearalenone (median 194 μg/kg), but no cis-zearalenone was detected.

(3S)-14,16-Dihy-droxy-3-methyl-3,4,5,6,9,10,11,12-octa-hydro-1H-2-benzoxacyclo-tetra-decine-1,7(8H)-dione (zearalanone) monohydrate.

The absolute configuration of the title compound, C(18)H(24)O(5)·H(2)O, was not been determined by anomalous-dispersion effects, but has been assigned by reference to an unchanging chiral centre in the synthetic procedure. Intra-molecular O-H⋯O hydrogen bonds stabilize the mol-ecular conformation. In the crystal, O-H⋯O hydrogen bonds link the main mol-ecules and the water mol-ecules, forming an infinite three-dimensional network.