Publications by authors named "Sarah Davidson-Fritz"
Article Synopsis
- Refining assumptions about the fraction absorbed (Fabs) can enhance the performance of pharmacokinetic models that use in vitro-to-in vivo extrapolation (IVIVE) methods for predicting oral bioavailability (Fbio) of chemicals.
- In this study, over 400 non-pharmaceuticals were tested for apparent permeability (Papp) using the Caco-2 cell line, leading to the development of a random forest quantitative structure-property relationship (QSPR) model which improved predictions of human bioavailability compared to rat data.
- The findings were integrated into a high throughput toxicokinetics (HTTK) framework to estimate equivalent doses for bioactivity based on in vitro data, resulting in only minor changes to exposure and bioactivity
High-Throughput Transcriptomics Screen of ToxCast Chemicals in U-2 OS Cells.
Toxicol Appl Pharmacol
October 2024
New approach methodologies (NAMs) aim to accelerate the pace of chemical risk assessment while simultaneously reducing cost and dependency on animal studies. High Throughput Transcriptomics (HTTr) is an emerging NAM in the field of chemical hazard evaluation for establishing in vitro points-of-departure and providing mechanistic insight. In the current study, 1201 test chemicals were screened for bioactivity at eight concentrations using a 24-h exposure duration in the human- derived U-2 OS osteosarcoma cell line with HTTr.
View Article and Find Full Text PDF'Cell Painting' is an imaging-based high-throughput phenotypic profiling (HTPP) method in which cultured cells are fluorescently labeled to visualize subcellular structures (i.e., nucleus, nucleoli, endoplasmic reticulum, cytoskeleton, Golgi apparatus / plasma membrane and mitochondria) and to quantify morphological changes in response to chemicals or other perturbagens.
View Article and Find Full Text PDFChemical risk assessment considers potentially susceptible populations including pregnant women and developing fetuses. Humans encounter thousands of chemicals in their environments, few of which have been fully characterized. Toxicokinetic (TK) information is needed to relate chemical exposure to potentially bioactive tissue concentrations.
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