Full Thickness EpiDerm: a dermal-epidermal skin model to study epithelial-mesenchymal interactions.

Unlike previous dermal-epidermal models, Full Thickness EpiDerm is cultured in an easily manipulated cell culture insert, and the tissue extends from wall to wall. In terms of ease of use, these characteristics greatly facilitate the testing of potential allergens or irritants in that direct topical application is possible. Topical exposure to the common surfactant, 1% Triton X-100, resulted in MTT tissue viability dose-response curves that fell within the normal range of the keratinocyte-only tissue, EpiDerm.