Validation of CRISPR activation system in cells using multicistronic plasmid vectors.

mosquitoes transmit several pathogens including flaviviruses to humans which result in high morbidity and mortality. Owing to adaptability and climate change, these mosquito vectors are predicted to establish in new geographical areas thus exposing larger populations to the risk of infection. Therefore, control of vector is necessary to prevent disease transmission.

The Inception of the "Two Trolley Technique" for Supine Positioning in a Patient With Penetrating Injury to the Back.

Penetrating trauma to the back causes the anesthesiologist many difficulties in airway management and obtaining central lines due to the inability to position supine. Lateral position intubation for the same has been described earlier but still remains unfamiliar. Here, we describe the case of a stab injury to the back and how we achieved the optimal supine position using the "Two Trolley Technique.

Spatial analysis of chronic obstructive pulmonary disease and its risk factors in an urban area of Trivandrum, Kerala, India.

Background: Chronic obstructive pulmonary disease (COPD) is the second leading cause of death in India. The objective of this study was to map COPD cases and its risk factors and to determine the association between them using geographic information system (GIS) in a semi-urban area of Trivandrum, South India.

Materials And Methods: This community-based cross-sectional, descriptive study (n = 494) was conducted in a subcenter area of a primary health center.

Impact of Right Ventricular Function on Left Ventricular Torsion and Ventricular Deformations in Pulmonary Artery Hypertension Patients.

Introduction: Ventricular interdependence in pulmonary arterial hypertension (PAH) by the use of most recent echocardiographic techniques is still rare. The current case-controlled study aims to assess left ventricular (LV) torsion in patients with PAH.

Methods: The study included 42 cases of moderate to severe PAH and 42 age and gender-matched healthy controls between March 2016 and January 2018.

Spatial analysis of hypothyroidism and ground water pH in an Urban Area of Kerala using the geographic information system.

Hypothyroidism is a disease assuming increasing relevance. The causative role of acidic nature of drinking water has not yet been investigated in Kerala. We attempted to determine the spatial association between the occurrence of self-reported hypothyroidism and pH of ground water using the geographic information system.

A cross-sectional study to assess prevalence and factors associated with mild cognitive impairment among older adults in an urban area of Kerala, South India.

Objectives: To assess the prevalence and factors associated with mild cognitive impairment (MCI) among older adults in an urban area of South India.

Setting: The study was conducted in the capital city of Thiruvananthapuram in the South Indian state of Kerala.

Participants: The study participants were community-dwelling individuals aged 60 years and above.

Seroprevalence of dengue infection in pregnant women and placental antibody transfer.

Background: Pregnant women and infants are vulnerable for developing severe dengue. This study was conducted to determine the seroprevalence of dengue infections among pregnant women, their offsprings and its association with outcomes.

Methods: A cross-sectional study was conducted among pregnant women, admitted for delivery in a tertiary mother and child hospital in Thiruvananthapuram.

Contamination of Household Open Wells in an Urban Area of Trivandrum, Kerala State, India: A Spatial Analysis of Health Risk Using Geographic Information System.

Objective: To assess the sanitary condition and water quality of household wells and to depict it spatially using Geographic Information System (GIS) in an urban area of Trivandrum, Kerala state, India.

Study Design: A community-based cross-sectional census-type study.

Methods: Study was conducted in an urban area of Trivandrum.

Role of Geographic Information System in Assessing Determinants of Cardiovascular Disease: An Experience From a Low- and Middle-Income Country.

Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in the world. The determinants of CVD in an urban population using conventional and geographic information system techniques were attempted as a community-based census-type cross-sectional study in Kerala, India, among 1649 individuals residing in 452 households. Sociodemographic details, risk factor exposures, and self-reported disease prevalence were determined.

Investigation of an outbreak of measles: failure to vaccinate or vaccine failure in a community of predominantly fishermen in Kerala.

Measles outbreaks continue to occur in developing countries. This study attempted to explore the context of an outbreak of measles in a community of predominantly fishermen in Kerala to find out whether the outbreak was the result of a failure to vaccinate or failure of the vaccine itself. A cross sectional study was conducted in Mukkola village of Thiruvananthapuram district, Kerala, India.

Detection of numerical chromosomal abnormalities in epithelial ovarian neoplasms by fluorescence in situ hybridization (FISH) and a review of the current literature.

Preliminary retrospective chromosomal analysis was performed using fluorescence in situ hybridization (FISH) with alphoid DNA probes for chromosomes 1, 3, 6, 8, 12, 17, and X. Twenty-four epithelial ovarian tumors were examined in this pilot study, including 8 borderline (LMP) serous tumors, 9 serous carcinoma, and 7 mucinous carcinoma. Hybridization signals were counted to demonstrate the frequency of aneusomy, trace chromosomal progression, and identify the predominance of chromosome copy number abnormalities that are specific to a particular histotype.