Publications by authors named "Sara Thabet"
Article Synopsis
- Bluetongue virus (BTV) causes bluetongue disease in ruminants and is transmitted mainly by biting midges.
- Recent discoveries have identified several atypical BTV serotypes that are generally asymptomatic and don't reproduce well in their insect carriers.
- This report highlights the detection of two atypical BTV strains in Gafsa, Tunisia: the recurring BTV-Y TUN2022 and a new strain called BTV-W TUN2022.
Background: The IS6110 insertion sequence, a member of the IS3 family of insertion sequences, was found to be specific to the Mycobacterium tuberculosis complex (MTBC). Although IS6110 has been extensively characterized as a transposable genetic marker, the evolutionary history of its own transposase-encoding sequence has not, to the best of our knowledge, been investigated.
Methodology/principal Findings: Here we explored the evolution of the IS6110 sequence by analysing the genetic variability and the selective forces acting on its transposase-encoding open reading frames (ORFs) A and B (orfA and orfB).
Objectives: Fingerprinting of Mycobacterium tuberculosis complex strains based on the IS6110 insertion sequence would considerably gain in terms of discriminatory power and versatility if both 5' and 3' polymorphisms were simultaneously targeted, and if it benefited from automated capillary electrophoresis. In response to these requirements, we developed IS6110-5'3'FP (IS6110 5' and 3' fluorescent polymorphisms).
Methods: IS6110-5'3'FP involves the construction of an M.