The dissipation and risk alleviation mechanism of PAHs and nitrogen in constructed wetlands: The role of submerged macrophytes and their biofilms-leaves.

The role of submerged macrophytes (Vallisneria natans, Hydrilla verticillata and artificial plant) and their biofilms-leaves for the dissipation and risk alleviation mechanism of PAHs (phenanthrene and pyrene) and nitrogen in constructed wetland systems with PAH-polluted sediments were investigated. Biofilms-leaves/surface might contribute to PAHs degradation, which was positively correlated with PAHs degrading bacteria. Nitrogen-fixing bacteria in biofilms on surface might cause total nitrogen in sediment (TNs) increasing by 4% from 14th d to 28th d indirectly when suffering PAHs pollution.

The responding and ecological contribution of biofilm-leaves of submerged macrophytes on phenanthrene dissipation in sediments.

The bacterial communities and ecological contribution of biofilm-leaves of the Vallisneria natans (VN), Hydrilla verticillata (HV) and artificial plant (AP) settled in sediments with different polluted levels of phenanthrene were investigated by high-throughput sequencing in different growth periods. There was no significant difference among the detected Alpha diversity indices based on three classification, attached surface, spiking concentration and incubation time. While Beta diversity analysis assessed by PCoA on operational taxonomic units (OTU) indicated that bacterial community structures were significantly influenced in order of attached surface > incubation time > spiking concentration of phenanthrene in sediment.