Multilayered films fabricated from plasmid DNA and a side-chain functionalized poly(beta-amino ester): surface-type erosion and sequential release of multiple plasmid constructs from surfaces.

Hydrolytically degradable polyamines can be used to fabricate multilayered polyelectrolyte films that erode and release DNA in aqueous environments. Past studies have investigated films fabricated from poly(beta-amino ester) 1 and the influence of polymer backbone structure on film erosion and the release of anionic polyelectrolytes. This investigation sought to characterize the influence of polymer side-chain structure on the stability of multilayered films in physiologically relevant media.