Development of a single-domain antibody to target a G-quadruplex located on the hepatitis B virus covalently closed circular DNA genome.

To achieve a virological cure for hepatitis B virus (HBV), innovative strategies are required to target the covalently closed circular DNA (cccDNA) genome. Guanine-quadruplexes (G4s) are a secondary structure that can be adopted by DNA and play a significant role in regulating viral replication, transcription, and translation. Antibody-based probes and small molecules have been developed to study the role of G4s in the context of the human genome, but none have been specifically made to target G4s in viral infection.

Perceptions of Supervision in an Outpatient Psychiatry Clinic.

Objectives: There is little guidance regarding best practices in supervision of psychiatric residents. As a result, expectations for both supervisors and trainees are often unclear. This study explored the experiences of trainees and supervisors in outpatient supervision, in order to identify areas for potential improvement.

Fomitiporia punctata (Basidiomycota, Hymenochaetales) and its presumed taxonomic synonyms in America: taxonomy and phylogeny of some species from tropical/subtropical areas.

The status of the F. punctata complex in tropical/subtropical America, and more specifically in Cuba and the surrounding area, was partially assessed. Fomitiporia langloisii, Fomitiporia dryophila and Fomitiporia maxonii, three names long considered taxonomic synonyms of F.