New species of Echinobothrium van Beneden, 1849 (Cestoda: Diphyllidea) from Indo-Pacific maskrays (Neotrygon Castelnau) off Australia and Borneo.

Four new diphyllidean species of the genus Echinobothrium van Beneden, 1849 are described from Indo-Pacific maskrays (Neotrygon Castelnau, Dasyatidae). Echinobothrium giraffaeous sp. n.

Coping with current impacts: The case of Scyliorhinus canicula in the NW Mediterranean Sea and implications for human consumption.

The small-spotted catshark (Scyliorhinus canicula) is a bottom-dwelling elasmobranch that represents the most discarded catch in terms of biomass in the Catalan coast (NW Mediterranean). Potential impacts affecting its population and food safety implications have been assessed in three localities along the Catalan coast. Distinct indicators were integrated, such as biological data, ingested anthropogenic items (plastic and cellulose-like items), parasitological indices, trace metal concentrations and histopathology using liver as target organ.

Revealing the capability of the European hake to cope with micro-litter environmental exposure and its inferred potential health impact in the NW Mediterranean Sea.

Prevalence, abundance, concentration, size and composition of anthropogenic items (AIs) (synthetic and non-synthetic) ingested by Merluccius merluccius juvenile specimens and from near-bottom water samples from different localities off the Catalan coast (NW Mediterranean), were characterized. The potential effect of AIs on fish condition was assessed through different health indicators. Virtually all AIs found in fish and near-bottom water samples were fibres.

Assessment of the health status of the European anchovy (Engraulis encrasicolus) in the NW Mediterranean Sea from an interdisciplinary approach and implications for food safety.

The European anchovy (Engraulis encrasicolus) is a small pelagic fish with an outstanding commercial value supporting important fisheries and is a key component of pelagic ecosystems in the Mediterranean Sea. Progressive reductions in the population size of this species has been observed in the Mediterranean Sea during recent decades, accompanied by a decline in the body condition, as well as the size/age of maturation. Nonetheless, the health status has not been yet assessed using a holistic approach.

Natural variability and potential use of melanomacrophage centres as indicators of pollution in fish species from the NW Mediterranean Sea.

The present study provides a baseline assessment of the prevalence and densities of splenic melanomacrophage centres (MMCs) in 18 fish species from the NW Mediterranean Sea related to spatiotemporal and environmental factors and fish traits. Their correlation with other established health indicators, such as body condition indices (condition factor, hepatosomatic and gonadosomatic indices), parasite community descriptors and histological assessment of target organs (gills, liver and spleen) is also assessed. Despite MMCs variability is mainly attributed to the species identity and fish size, their potential use as generic biomarkers of health condition is pointed out for certain species (e.

New morphological and molecular perspectives about (Acanthocephala: Oligacanthorhynchidae) from wild boar, Linn., in Ukraine.

We make new morphological observations not previously reported for the old acanthocephalan Macracanthorhynchus hirudinaceus (Pallas, 1781) Travassos, 1917, described for the first time about 240 years ago. Our specimens were collected from the wild boar, Sus scrofa Linn., in Ukraine in 2005.

Morphological and molecular description of a distinct population of Echinorhynchus gadi Zoega in Müller, 1776 (Paleacanthocephala: Echinorhynchidae) from the pacific halibut Hippoglossus stenolepis Schmidt in Alaska.

Purpose: Echinorhynchus gadi is one of the most widely distributed and commonly described acanthocephalans in marine fishes throughout the world. We provide a detailed morphometric and molecular description of a distinct Alaska population collected from the Pacific halibut Hippoglossus stenolepis Schmidt (Pleuronectidae) compared to those from other hosts and regions, illustrating new features never previously reported.

Methods: We described new specimens by microscopical studies, augmented by SEM, Energy Dispersive x-ray and molecular analyses, and histopathology.

Multi-organ characterisation of B-esterases in the European sea bass (Dicentrarchus labrax): Effects of the insecticide fipronil at two temperatures.

In fish, the study of cholinesterases (ChEs) and carboxylesterases (CEs), apart from their involvement in neural activity and xenobiotic metabolism, respectively, requires to be further explored. The European sea bass (Dicentrarchus labrax) was the fish model used to characterise B-esterases in several matrices and organs, as well as to assess the impacts of the insecticide fipronil at two temperatures: the natural temperature at the time of sampling (13 °C) and at 16 °C (based on climate change-related predictions for the Mediterranean region). Fipronil exerts harmful effects in non-target species; however, some countries are reluctant to implement regulations without additional evidence on their toxicity.

Morphological and Molecular Description of Soota and Bhattacharya, 1981 (Acanthocephala: Rhadinorhynchidae) from Marine Fish off the Pacific Coast of Vietnam.

Soota and Bhattacharya, 1981 , has remained unknown since its original incomplete description from 2 male specimens collected from the flat needlefish Valenciennes (Belonidae) off Trivandrum, Kerala, India. Recent collections of fishes along the Pacific coast of Vietnam in 2016 and 2017 produced many specimens of the same species from the striped bonito Temminck and Schlegel (Scombridae) off the southern Pacific coast of Vietnam at Nha Trang. We describe females for the first time, assign a female allotype status, and provide an expanded description of males from a larger collection completing missing information on hooks and hook roots, receptacle, lemnisci, cement glands, Saefftigen's pouch, and trunk spines.

Multibiomarker approach to fipronil exposure in the fish Dicentrarchus labrax under two temperature regimes.

Fipronil is a phenylpyrazole insecticide widely used to control pests in agriculture even though evidence of harmful side effects in non-target species has been reported. A comprehensive study on the effects of dietary administration of Regent®800WG (80 % fipronil) in European sea bass juveniles was carried out under two temperature regimes: a) natural conditions, and b) 3 °C above the natural temperature (an increase predicted for the NW Mediterranean by the end of this century). Fipronil was added to the fish food (10 mg fipronil /Kg feed) and the effects were studied at several time points including right before administration, 7 and 14 days after daily fipronil feed and one-week after the insecticide withdrawal from the diet (depuration period).

Drivers of parasite community structure in fishes of the continental shelf of the Western Mediterranean: the importance of host phylogeny and autecological traits.

We explored the relationships between features of host species and their environment, and the diversity, composition and structure of parasite faunas and communities using a large taxonomically consistent dataset of host-parasite associations and host-prey associations, and original environmental and host trait data (diet, trophic level, population density and habitat depth vagility) for the most abundant demersal fish species off the Catalonian coast of the Western Mediterranean. Altogether 98 species/taxa belonging to seven major parasite groups were recovered in 683 fish belonging to 10 species from seven families and four orders. Our analyses revealed that (i) the parasite fauna of the region is rich and dominated by digeneans; (ii) the host parasite faunas and communities exhibited wide variations in richness, abundance and similarity due to a strong phylogenetic component; (iii) the levels of host sharing were low and involved host generalists and larval parasites; (iv) the multivariate similarity pattern of prey samples showed significant associations with hosts and host trophic guilds; (v) prey compositional similarity was not associated with the similarity of trophically transmitted parasite assemblages; and (vi) phylogeny and fish autecological traits were the best predictors of parasite community metrics in the host-parasite system studied.

Morphological and molecular description of Rhadinorhynchus laterospinosus Amin, Heckmann & Ha, 2011 (Acanthocephala, Rhadinorhynchidae) from marine fish off the Pacific coast of Vietnam.

Rhadinorhynchus laterospinosus Amin, Heckmann & Ha, 2011 (Rhadinorhynchidae) was described from a single female collected from a trigger fish, Balistes sp. (Balistidae) from the northern Pacific coast of Vietnam in Halong Bay, Gulf of Tonkin. More recent collections of fishes in 2016 and 2017 revealed wider host and geographical distributions.

Species of Lepidapedon Stafford, 1904 (Digenea: Lepidapedidae) from deep-sea fishes in the Western Mediterranean: molecular and morphological evidence.

The species diversity of Lepidapedon Stafford, 1904 (Lepidapediae) in the Western Mediterranean was assessed based on samples from five deep-sea gadiform fishes collected between the Balearic Islands and the Catalonian coast of Spain: Lepidion lepidion (Risso) and Mora moro (Risso) (both Moridae); Coelorinchus mediterraneus Iwamoto & Ungaro and Trachyrincus scabrus (Rafinesque) (both Macrouridae); and Phycis blennoides (Brünnich) (Phycidae). Integration of morphological and molecular evidence (28S rRNA gene and the mitochondrial nad1 gene) revealed that the deep-sea fishes in the Western Mediterranean share two species of Lepidapedon. Lepidapedon desclersae Bray & Gibson, 1995 was recovered in all five fish species of three families [Moridae (L.

Preliminary results on the uptake and biochemical response to water-exposure of Tamiflu® (oseltamivir phosphate) in two marine bivalves.

Tamiflu® (oseltamivir phosphate, OST) is an antiviral drug used for the pandemic treatment of avian influenza but few data are available regarding its toxicity. It should be noted that acute adverse responses are not likely to occur due to low environmental presence of this drug. Nonetheless, water concentration levels of this compound may reach the µg/L range under influenza episodes.

Multibiomarker biomonitoring approach using three bivalve species in the Ebro Delta (Catalonia, Spain).

Bivalves have proved to be useful bioindicators for environmental pollution. In the present study, mussels (Mytilus galloprovincialis), cockles (Cerastoderma edule), and razor shells (Solen marginatus) were collected in the Ebro Delta, an extensive area devoted to rice farming and affected by pesticide pollution, from April to July, the heaviest rice field treatment period. Possible effects of pollution were assessed through biochemical markers (carboxylesterase (CE), antioxidant and neurotoxicity-related enzymes, and lipid peroxidation levels).

A new species of Hamaticolax (Copepoda: Bomolochidae) from Helicolenus dactylopterus (Delaroche, 1809) (Scorpaeniformes: Sebastidae) in NW Mediterranean deep waters and notes on patterns of host use and host-specificity of the genus.

Hamaticolax juanji n. sp. is described from specimens collected from the blackbelly rosefish Helicolenus dactylopterus Delaroche 1809 (Scorpaeniformes: Sebastidae).

Revision of the family Sphyriocephalidae Pintner, 1913 (Cestoda: Trypanorhyncha), with the description of Heterosphyriocephalus encarnae n. sp. and redescriptions of two species of Sphyriocephalus.

The family Sphyriocephalidae Pintner, 1913, which comprises the genera Hepatoxylon Bosc, 1811, Sphyriocephalus Pintner, 1913 and Heterosphyriocephalus Palm, 2004, is revised from newly-collected and museum material. Heterosphyriocephalus encarnae n. sp.

Morphological and molecular characterisation of Ditrachybothridium macrocephalum Rees, 1959 (Cestoda: Diphyllidea) from Galeus melastomus Rafinesque in the Western Mediterranean.

New morphological, molecular and ecological data for Ditrachybothridium macrocephalum Rees, 1959 (Cestoda: Diphyllidea) are presented and discussed based on specimens recovered from the blackmouth catshark Galeus melastomus Rafinesque (Scyliorhinidae) in the Western Mediterranean. A redescription of the plerocercus of this parasite is provided and new data on immature and mature worms including the first description of the eggs are reported, based on light and scanning electron microscopy observations. Analysis of 28S rDNA (domains D1-D3) sequences from plerocerci, immature and adult specimens revealed that they are conspecific with specimens from the North East Atlantic.

A new species of Bathycreadium Kabata, 1961 (Digenea: Opecoelidae) from Phycis blennoides (Brünnich) (Gadiformes: Phycidae) in the western Mediterranean.

Bathycreadium brayi n. sp. is described based on specimens collected from the deep-sea greater forkbeard Phycis blennoides (Brünnich) (Gadiformes: Phycidae) in the Western Mediterranean.

Morphometric and molecular characterisation of specimens of Lepidapedon Stafford, 1904 (Digenea: Lepidapedidae) from the deep-sea fish Mora moro (Risso) (Teleostei: Moridae) in the western Mediterranean.

In a study of the parasites of the deep-sea fish Mora moro (Risso) (Gadiformes: Moridae) off the Mediterranean coasts of Catalonia and the Balearic Islands (Spain), we were able to distinguish two morphs of specimens belonging to Lepidapedon Stafford, 1904 (Digenea: Lepidapedidae). This material is herein described and illustrated. Comparative sequence analyses using partial mitochondrial nad1 sequences revealed that the material assigned to one of these morphs can be considered conspecific with the material identified as Lepidapedon desclersae Bray & Gibson, 1995 from the same host.