Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations.

Aim: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared.

Materials & Methods: 'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed.

Results: Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance.

Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line.

With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-MB 231, a human breast cancer cell line. The synthesized ASNPs were characterized by spectroscopic methods.

Novel Structural Changes during Temperature-Induced Self-Assembling and Gelation of PLGA-PEG-PLGA Triblock Copolymer in Aqueous Solutions.

The thermoresponsive amphiphilic block copolymer poly(d,l-lactic acid-co-glycolic acid)-block-poly(ethylene glycol)-block-poly(d,l-lactic acid-co-glycolic acid) (PLGA-PEG -PLGA), which exhibits a reversible temperature-induced sol-gel transition at higher polymer concentrations in aqueous solution has attached a great deal of interest because of its potential in biomedical applications. In the present work, the length of the hydrophobic PLGA blocks is kept constant, whereas the length of the hydrophilic PEG block is altered and this variation has a pronounced impact on the phase behavior of the aqueous samples and the structure of the polymer. A short PEG block promotes gelation at a low temperature, whereas a longer PEG block shifts the gelation point to higher temperature.

Characterization of oligosaccharide-functionalized hyperbranched poly(ethylene imine) and their complexes with retinol in aqueous solution.

Structure, internal density distribution, and size of hyperbranched poly(ethylene imine) (PEI) functionalized with various amounts of maltose (PEI-Mal) in phosphate buffer were studied by small angle X-ray scattering (SAXS) and dynamic light scattering (DLS). The value of pH was varied in the range from 3 to 9. Virtually no effect of pH on the nanostructure was found in this interval.