Validated High Performance Liquid Chromatographic Method for Stability Study of Eptifibatide.

Eptifibatide (EPT) is a cyclic heptapeptide derived from a protein found in the venom of the south-eastern pygmy rattle snake used as an antiplatelet drug. In this study, a newly developed HPLC method demonstrating no interference from the different degradation products of EPT has been optimized and validated. The method was based on HPLC separation of eptifibatide from its degradation products using reversed phase C18 column at ambient temperature with mobile phase consisting of acetonitrile: 50 mM sodium dihydrogen orthophosphate dihydrate, pH was adjusted to 2.