[On the issue of cooperation in organizing the laboratory medicine education].

In the Omsk public medical Academy, the informational and technological support of laboratory medicine education at undergraduate and postgraduate stages required to organize the cooperation between the Russian Association of medical laboratory diagnostics and the Academy course of clinical diagnostics. The non-commercial interaction was organized between the Academy and the manufacturers and suppliers of laboratory diagnostic systems. This approach permitted to implement the cycle of scientific educational forums and to start the territorial program of quality management in laboratory research.

Triple-site antigen capture ELISA for human myoglobin can be more effective than double-site assay.

Using a panel of monoclonal antibodies against human myoglobin (Mb), we have shown that the sensitivity of antigen-capture ELISA can be significantly increased by simultaneous immobilization of two cooperating capture monoclonal antibodies on a solid phase. This method ("triple-site ELISA") uses three monoclonal antibodies to different epitopes of the same antigen (two capture/one tracer) unlike the traditional double-site assay using one capture and one tracer monoclonal antibody. We developed double- and triple-site ELISA for Mb by varying the capture and tracer monoclonal antibodies.

[The role of the prostaglandin system in the cardioprotective effect of adaptation to hypoxia in stress].

The adaptation to periodic altitude hypoxia is known to have cardioprotective and antiarrhythmic effects in stress-induced and ischemic lesions. The effects are assumed to be associated with the enhanced activity of the body's stress-limiting systems, including prostaglandins (PG). Wistar rats were adapted in a hypobaric chamber at an altitude of 4000 m for 6 hours during 40 days.

[The concentrations of somatotropic hormone and insulin in the blood of heart surgery patients with a complicated course of the postoperative period].

It has been shown that the course of the early postoperative period in cardiosurgical patients and its outcome is to a great extent related to adequate balance of adaptation hormones, whose levels and changes are determined by the functional state of compensatory-adaptive systems. Patients with favourable outcome of the complicated postoperative period along with synchronous activation of sympathoadrenal system (SAS), hypophyseoadrenal system (HAS) and an elevated somatotropic hormone (STH) level demonstrated adequately high blood insulin content, with the equilibrium in adrenalin/insulin and cortisol/insulin ratios retained, and moderate STH predominance over insulin. In 1/4 of patients with complicated postoperative period and unfavourable outcome an attenuated SAS response was accompanied by excessively high STH, ACTH, cortisol blood content and a lower insulin level, which determined relative insulin insufficiency.

[Relations of catecholamine and prostaglandin contents in the blood of rats exposed to acute stress and during adaptation to stress].

In Wistar male rats, 1-h acute immobilization stress resulted in the increased plasma adrenalin, noradrenaline and dopamine in approximately 13, 8 and 23 times, respectively. Plasma PGF2 alpha increased in 3.5 times while PGE remained unchanged; this resulted in the decreased PGE/PGF2 alpha relation in 2.

[Epicardial destruction of the bundle of Kent by pulsating electric current and the criteria of its effectiveness in Wolff-Parkinson- White syndrome].

The article deals with a new method of treatment of the Wolff-Parkinson-White syndrome. The operation for epicardial destruction of Kent's bundle by pulsating current was developed by the authors. It is distinguished by its simplicity and the possibility to determine the efficacy of the procedures during the operation itself.

[The course of the early postoperative period in heart surgery patients in relation to the state of humoral regulatory mechanisms].

It has been shown that the course and outcome of the early postoperative period in cardiosurgical patients are to a great extent related to the functioning of the adaptation systems under study and their interrelations. An essential role of sympathoadrenal system (SAS) in the control over hormonal body status has been established. It ensures, provided its activity is retained, interaction of other hormonal systems as a uniform functional mechanism in postoperative stress.

[Thyroid hormone content of the blood plasma and tolerance for physical loading in ischemic heart disease patients].

Blood thyroid hormones were measured before and after the rationed exercise test in 79 patients with angiographically verified diagnosis of coronary heart disease. A relationship was demonstrated between postexercise blood T3 variation and functional coronary circulation reserve. Hormonal baselines, and exercise-induced blood T4 changes were basically similar in patients with coronary disease of different severity.

[The scissors phenomenon in the reaction of hormonal systems to the physical load test in ischemic heart disease patients].

A difference between baseline and final plasma hormonal (insulin, cortisol, somatotrophic hormone, triiodothyronine (T3) and thyronine (T4) levels, related to the pattern of response to physical stress, was demonstrated in 67 coronary patients with angiographically verified diagnosis. Post-exercise increase in these hormones was associated with their lowered baseline and elevated final blood levels, as compared to those patients who showed a decrease in hormonal levels in response to exercise, with the baselines exceeding final values.

Isoenzyme spectrum of creatine kinase and lactate dehydrogenase in the myocardium of patients with ischaemic heart disease.

The purpose of the study was to find out the character of the restructuring of enzymes and isoenzymes of creatine kinase (CK), lactate dehydrogenase (LDH) and glucose-6-phosphate-dehydrogenase (G 6 P-DH) in the myocardium, which occurs in patients with ischaemic heart disease (IHD), and to compare the activity of the aforementioned enzymes in the myocardium of patients with IHD, examined intra vitam, and in patients who died suddenly. An analysis was made of 11 samples obtained by myocardial biopsy from the left ventricle of patients with IHD and of 11 samples obtained at autopsy of patients with IHD who have died a sudden death. Serving as controls was bioptic material from the myocardium of 12 patients without IHD.

[Evaluation of the status of the myocardium during surgical correction of congenital heart defects by simultaneous study of ultrastructure and macroergic phosphates (in biopsies of the human heart)].

During surgical correction of Fallot's tetrad the authors discovered the decrease of macroergic phosphate concentration and moderately marked signs of cardiomyocytic ultrastructural changes, such as mitochondrial injuries, decrease of glycogen levels, insignificant intracellular edema, no increase of membrane penetration. Changes of endothelial cells were absent. In children not over 1 year old under myocardial protection with hypothermic perfusion (at low volume rates) some decrease of macroergic phosphates occurred, as well as a moderate decrease of the mitochondrial index, glycogen content decrease, there was no intracellular edema.

[Atherosclerotic lesion of the coronary arteries in ischemic heart disease patients with differing levels of insulin, cortisol and somatotropic hormone and the type of reactions of these hormones to physical loading].

Plasma insulin, cortisol and somatotropic hormone (STH) levels of coronary patients are related to the extent of atherosclerotic lesion of coronary arteries. The relationship between coronary atherosclerosis and hormonal activity becomes particularly evident through the pattern of hormonal response (a rise or a fall) to rationed exercise. An elevated insulin baseline and reduced postexercise levels are typical for coronary patients with only slightly narrowed (25% or less) coronary arteries.

[Thyroid hormone content of the blood plasma and functional state of the myocardium in patients with chronic forms of ischemic heart disease].

Sixty-seven patients with angiographically-confirmed coronary heart disease (CHD) were investigated. Three patterns of thyroid hormone (T3 and T4) response to exercise were identified: rising, falling and unchanged levels. Coronary patients with rising T3 levels in response to exercise typically showed smaller left-ventricular ejection values as compared to those patients whose T3 levels declined.

[Comparison of the indices of left ventricular pumping function, contractility, myocardial metabolism and the magnitude of the R wave on the ECG of ischemic heart disease patients].

Central hemodynamics, myocardial contractility and consumption of major energy substrates (lactate, glucose, non-esterified fatty acids, NEFA), as well as the total R wave amplitude from 12 standard leads were assessed in 20 patients with neurocirculatory dystonia (the control group) and 36 patients with chronic coronary disease. The changes in central hemodynamics and contractility were particularly marked in 12 of the more severe coronary cases with a raised total R wave amplitude. Myocardial energy supply in such patients is provided at the expense of NEFA.

[Effectiveness of protecting the myocardium against ischemia with a normothermic cardioplegic solution and creatine phosphate].

The protective effects of cardioplegic solutions (CS) containing creatine phosphate (CP) were studied in a rat heart model of cardiopulmonary bypass and ischemic cardiac arrest. Isolated rat hearts were subjected to a 3-minute coronary infusion with CS containing CP in normothermic (37 degrees C) and hypothermic (4-6 degrees C) regimes. In the normothermia group, the postischemic functional recovery was 70-75% of the preischemic control value, while the cellular ATP and CP content was reduced but insignificantly.

[Correlation between serum lipid level and coronary bed lesions in patients with ischemic heart disease].

The blood lipid levels were collated with the atherosclerotic damage to the coronary bed in 124 patients with coronary heart disease. An increase in the content of total cholesterol and low density lipoprotein cholesterol directly correlated with the degree of the total lesion of the coronary bed. The concentrations of high density cholesterol and very low density cholesterol and the atherogenicity index showed no correlation with the severity of atherosclerotic changes in the coronary arteries.