Development of a syringe-hosted load-and-read immunoassay device using autoinjected distance readout in paper inserts.

Pressure-based signal readout is promising for developing instrument-free immunoassays, but new detection methods are desirable to further advance the applicability of point-of-care testing (POCT). Herein, we developed a syringe-hosted load-and-read immunoassay device using autoinjected distance readout in paper inserts. The device was composed of multiple disposable syringes as the host for immuno-recognition, a three-dimensional (3D)-printed loading magazine of the syringes, and paper inserts to the magazine for distance signal readout.

Development and Evaluation of a Noninvasive Microfluidic-Based Paper Analytical Device for Leptospirosis Diagnosis.

Leptospirosis is a re-emerging infectious disease that presents a diagnostic enigma for clinicians with frequent misdiagnosis due to lack of rapid and accurate diagnostic tests, as the current methods are encumbered by inherent limitations. The development of a diagnostic sensor with a sample-in-result-out capability is pivotal for prompt diagnosis. Herein, we developed a microfluidic paper-based analytical device (spin-μPAD) featuring a sample-in-result-out fashion for the detection of specific urinary biomarker, sph2 sphingomyelinase, crucial for noninvasive point-of-care testing.

Diagnosis of Neglected Tropical Zoonotic Disease, Leptospirosis in a Clinical Sample Using a Photothermal Immunosensor.

Photothermal biosensing based on nanomaterials has gained increasing attention because of its universality and simplicity. Diagnostics of neglected tropical diseases (NTDs) in low-resource settings are challenging in terms of speed, accuracy, and cost-effectiveness. By exploiting the photothermal property of carbon nanotubes (CNTs), simple thermometric measurements can be used to generate quantitative biochemical readouts.

Towards CRISPR powered electrochemical sensing for smart diagnostics.

Even though global health has been steadily improved, the global disease burden associated with communicable and non-communicable diseases extensively increased healthcare expenditure. The present COVID-19 pandemic scenario has again ascertained the importance of clinical diagnostics as a basis to make life-saving decisions. In this context, there is a need for developing next-generation integrated smart real-time responsive biosensors with high selectivity and sensitivity.

Early detection of leptospirosis using Anti-LipL32 carbon nanotube immunofluorescence probe.

Leptospirosis is a widespread zoonosis and an emerging public health problem. Leptospirosis symptoms are often confused or misdiagnosed with other febrile illness like malaria, viral hepatitis, influenza, dengue, typhoid, melioidosis, and scrub typhus as the clinical manifestations are almost similar. Therefore, early and accurate diagnosis of leptospirosis is indeed critical for proper and prompt treatment.