New Mutations of the ID1 Gene in Acute Myeloid Leukemia Patients.

Objectives: Overexpression of the inhibitor of DNA binding 1 (ID1) protein is found in many types of cancer. In acute myeloid leukemia (AML), the expression of ID1 is induced by abnormal tyrosine kinases, such as FLT3 and BCR-ABL. High level expression of ID1 is associated with poor prognosis in young patients.

Prevalence and gB genotype distribution of human cytomegalovirus among HIV sero-negative and HIV sero-positive orphans in Thailand.

Background: Human Cytomegalovirus (HCMIV infects humans in all geographic areas. Polymorphisms ofglycoprotein B (gB) have been usedforgenotypic characterization of HCMV However information of gB genotyping of HCMV in Thailand is not clearly known especially in children.

Material And Method: A cross-sectional study was conducted to assess HCMV infection in 236 HIV seronegative and HIV seropositive children who attended an orphanage in Nonthaburi, Thailand by nested-PCR technique using urine specimens.

Human cytomegalovirus gB1 genotypes among children who live at the Phayathai Babies' home in Nonthaburi, Thailand.

We conducted a survey of human cytomegalovirus (HCMV) genotypes among 176 children aged 1 month to 5 years living at Phayathai Babies' Home in Nonthaburi Province, Thailand to determine the prevalence of HCMV glycoprotein B (gB) genotype. The study was conducted on urine samples using nested polymerase chain reaction and restriction fragment length polymorphism; the HCMV gB1 genotype was found in 89% of subjects, much higher than previous reports. Our results show a high proportion of HCMV gB1 infected children in this population.

The Effect of a Single Nucleotide Substitution in the Splicing Silencer in the tat/rev Intron on HIV Type 1 Envelope Expression.

A complex mRNA splicing pattern, which remains to be fully characterized, influences HIV-1 gene expression. In this study, poor envelope expression of a primary HIV-1 isolate was observed and linked to increased splicing of the two coding exons of tat/rev. The substitution of a nucleotide G, located 28 nucleotides upstream of the splice acceptor site SA7 in the recently identified intron splicing silencer sequence, was found to be responsible for the poor envelope expression.

Effect of amino acid substitution of the V3 and bridging sheet residues in human immunodeficiency virus type 1 subtype C gp120 on CCR5 utilization.

The V3 loop and the bridging sheet domain of human immunodeficiency virus type 1 (HIV-1) subtype B envelope glycoprotein gp120 have been implicated in CCR5 coreceptor utilization. In this study, mutant envelope glycoproteins of a subtype C isolate containing substitutions in the V3 or C4 region were generated to determine which are required for efficient CCR5-dependent cell fusion and viral entry. We found that the V3 crown and C4 residues are relatively dispensable for cell-cell fusion, although some residues may be involved in the regulation of early postentry steps in viral replication.