Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line.

Purpose: To present the properties of a newly developed immortalized human conjunctival epithelial cell (iHCjEC) line.

Methods: iHCjECs were developed to induce Simian Virus 40 large T-antigen (SV40LT) by incorporating lentivirus in a tetracycline (Tet)-regulated gene-expression system into primary cultures of human conjunctival epithelial cells. The population doubling time and morphology of the iHCjECs were analyzed.

Combined effect of coffee ingestion and repeated bouts of low-intensity exercise on fat oxidation.

We investigated the effect of the combination of coffee ingestion and repeated bouts of low-intensity exercise on fat oxidation. Subjects were seven young, healthy male adults. They performed four trials: a single 30-min bout of exercise following ingestion of plain hot water (WS) or coffee (CS); a trial with three 10-min bouts of exercise separated by 10-min periods of rest following ingestion of plain hot water (WR) or coffee (CR).

Horizontal intracorneal swirling water migration indicative of corneal endothelial function.

Purpose: To test our hypothesis about whether there is water migration in the horizontal corneal plane and investigate its developmental mechanism.

Methods: A fluorescein solution was intrastromally injected into normal and edematous corneas of rabbits, and the movement of the fluorescein solution was observed and recorded over time.

Results: In normal corneas, the water flow was characterized by a swirling movement from the center to the periphery in the stroma.