Quantification of levetiracetam in plasma and urine and its application to a pharmacokinetic study of traumatic brain injury patients.

Levetiracetam is an antiepileptic drug used to prevent or treat seizure in patients with severe traumatic brain injury. This study aimed to develop and validate methodology suitable for measuring levetiracetam concentrations in human plasma and urine. Plasma or urine (10 μl) samples were spiked with [H]-levetiracetam and processed using an acetonitrile precipitation.