The genome of the solitary bee Tetrapedia diversipes (Hymenoptera, Apidae).

Tetrapedia diversipes is a Neotropical solitary bee commonly found in trap-nests, known for its morphological adaptations for floral oil collection and prepupal diapause during the cold and dry season. Here, we present the genome assembly of T. diversipes (332 Mbp), comprising 2,575 scaffolds, with 15,028 predicted protein-coding genes.

Clinical performance of different adhesion strategies in non-carious cervical lesion restorations: A four-year randomized clinical trial.

Objective: To evaluate the retention rate and other clinical criteria of four different restorative techniques for non-carious cervical lesions (NCCLs) after 4 years.

Methods: This is a prospective, randomized, double-blind, and split-mouth study evaluating four different adhesion strategies in non-carious cervical lesion restorations: adhesive restorative system (Scotchbond Universal Adhesive/Filtek Z350XT) without (SBU) and with selective enamel acid-etching (E-SBU), resin-modified glass-ionomer cements (Vitremer; RMGIC), and ethylenediaminetetraacetic as acid pretreatment (E-RMGIC). In total, 200 restorations were placed in 50 patients.

Dissemination and Implementation - A Primer for Accelerating "Time to Translation" in Radiation Oncology.

The field of radiation oncology has achieved significant technological and scientific advancements in the 21 century. Yet uptake of new evidence-based practices has been heterogeneous, even in the presence of national and international guidelines. Addressing barriers to practice change requires a deliberate focus on developing and testing strategies tailored to improving care delivery and quality, especially for vulnerable patient populations.

A sustainable extraction approach for recovery of Rhamnogalacturonan I enriched pectin from Amazonian cocoa pod husk.

Approximately 16,000 thousand tonnes of cocoa husk are produced annually worldwide during the chocolate production chain, representing considerable potential for pectin extraction. The aim of this study was to investigate a new extraction technique using a binary system composed of carbon dioxide and water (CO + HO) at high pressure and temperature, on cocoa sample from floodplain ecosystem. Temperatures of 393.

Haemorrhagic Shock in a Pregnant Patient and the Unsuspicious Subclavian Catheter.

Introduction: Central venous catheterisation (CVC) is a commonly performed procedure in clinical practice. Although usually safe, complications can arise such inadvertent vascular lesion. This report is of a case of left brachiocephalic vascular lesion due to a subclavian catheter, thus raising awareness about this potential complication, which is not always immediately recognised.

A new species of Athyreus MacLeay, 1819 (Coleoptera: Geotrupidae: Bolboceratinae: Athyreini) from northeastern Brazil.

Athyreus arretado Celante & Carvalho, new species (Coleoptera: Geotrupidae: Bolboceratinae: Athyreini) is described from Casa Nova, Bahia, Brazil. An updated identification key to males in the Athyreus aeneus species group is provided. Distribution maps of some Bolboceratinae species with new records in northeastern Brazil for Athyreus tuberifer Felsche, 1909 from Sergipe; Neoathyreus (Neoathyreus) anthracinus (Klug, 1843) and Neoathyreus (Neoathyreus) lanuginosus (Klug, 1843) from Paraíba; Athyreus vavini Boucomont, 1902, Neoathyreus (Neoathyreus) braziliensis Howden, 1985, and Neoathyreus (Neoathyreus) centromaculatus (Felsche, 1909) from Pernambuco; and Bolbapium caesum (Klug, 1843) from Bahia are provided.

Morphology and molecular evidence reveal hidden diversity among snapping shrimp of the Alpheus obesomanus group (Decapoda: Alpheidae) with the description of a new species from Brazil.

The snapping shrimp family Alpheidae Rafinesque, 1815 includes numerous species, most of which present controversial geographical distributions. The disjunct distribution of Alpheus simus Guérin-Méneville, 1856 in the western Atlantic, from Florida to the south of the Caribbean Sea and then from Rio Grande do Norte to Bahia in Brazil, suggests that Brazilian material may belong to an undescribed species. The examination of specimens previously identified as A.

Wavelet-based time-frequency intermuscular beta-band coherence decreases with age but increases after mental fatigue in ankle muscles during gait independent of age.

Mental fatigue can affect cognitive function and interfere with motor performance. We examined if mental fatigue affected gait through age-specific modulation of wavelet-based time-frequency intermuscular beta-band coherence in muscles while walking on a treadmill at 1.2 m·s-1.

Adherence to Registration and Selective Outcome Reporting in Randomized Clinical Trials Published in Endodontic Journals Over the Past 5 Years: A Meta-Research Study.

Introduction: Prospective registration of randomized clinical trials (RCTs) is highly recommended to ensure research transparency and prevent selective outcome reporting (SOR). This study aimed to evaluate the adherence to registration and the presence of SOR in RCTs published in endodontic journals over the past 5 years.

Methods: Electronic searches were conducted in PubMed and the libraries of the Journal of Endodontics, International Endodontic Journal, European Endodontic Journal, and Australian Endodontic Journal.

Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape.

Breast cancer (BC) is a leading cause of death worldwide, particularly also among African woman. In order to better stratify patients for the most effective (immuno-) therapy, an in depth characterization of the immune status of BC patients is required. In this study, a cohort of 65 Ethiopian patients with primary BC underwent immune profiling by multicolor flow cytometry on peripheral blood samples collected prior to surgery and to any other therapy.

Effect of a constant rate infusion of ketamine on left ventricular systolic and diastolic function in dogs anesthetized with propofol.

Objective: To evaluate the effect of a constant rate infusion of ketamine on left ventricular systolic and diastolic function in dogs anesthetized with propofol.

Study Design: Prospective randomized, blinded clinical study.

Animals: Sixteen healthy dogs.

Improved production of palitantin by design of experiments and semi-synthesis of palitantin derivatives for bioactivity assessment.

A new method for the production and isolation of (+)-palitantin (1) is herein reported, from cultures of the fungal strain Penicillium sp. AMF1a. (+)-Palitantin was isolated in 160 mg/L yield, as an alternative procedure to obtain 1 at a larger scale.

Left Atrial Wall Thickness Measured by a Machine Learning Method Predicts AF Recurrence After Pulmonary Vein Isolation.

Background: Left atrial (LA) remodeling plays a significant role in the progression of atrial fibrillation (AF). Although LA wall thickness (LAWT) has emerged as an indicator of structural remodeling, its impact on AF outcomes remains unclear. We aimed to determine the association between LAWT and AF recurrence after pulmonary vein isolation (PVI), as well as to evaluate the relationship between LAWT and LA fibrosis.

Anaesthesia for hybrid revascularisation and valve replacement in a high-risk patient.

Hybrid procedures offer a promising solution for complex cases where open surgery is high risk, relying on the collaboration of anaesthesiologists, cardiac surgeons and interventional cardiologists. We present a male patient in his 70s with severe aortic stenosis, coronary artery disease, ascending aortic aneurysm and heart failure who underwent a hybrid cardiac procedure, including transcatheter aortic valve implantation, percutaneous coronary intervention of the right coronary artery and coronary artery bypass graft surgery. Through this hybrid intervention, involving a minimally invasive transcatheter and an open surgical procedure, the patient remained haemodynamically stable with minimal blood loss.

Hatching Egg Sanitizers Based on Essential Oils: Microbiological Parameters, Hatchability, and Poultry Health.

Eggshell contamination threatens the viability of hatching eggs. This contamination can be caused by harmless, opportunistic, or pathogenic bacteria. Although necessary, the use of synthetic antibiotics to treat eggshells can present several significant problems: They can be toxic and damage the shell, and, most worryingly, they can lead to bacterial resistance.

Synergistic Effects of Dietary Tryptophan and Dip Vaccination in the Immune Response of European Seabass Juveniles.

Vaccination is an effective, cost-efficient method to preventing disease outbreaks. However, vaccine procedures can induce adverse reactions due to stress, increasing plasma cortisol in the short term. In this context, tryptophan may prove to be fundamental as it has been demonstrated to have various desirable neuroendocrine attributes in different fish species.

Adolescents' Knowledge and Misconceptions About Sexually Transmitted Infections: A Cross-Sectional Study in Middle School Students in Portugal.

: Adolescence represents a period of heightened vulnerability to risky sexual behaviours. In Portugal, adolescents are initiating sexual activity at increasingly younger ages, making it relevant to characterise their knowledge about sexually transmitted diseases and prevention methods at an earlier stage of their development. : A cross-sectional observational study was conducted involving the application of a questionnaire to 750 ninth-grade students from Vila Nova de Gaia, Porto, Portugal.

Salivary Metabolomics in Patients with Long COVID-19 Infection.

Long COVID-19 has been characterized by the presence of symptoms lasting longer than 4 weeks after the acute infection. The pathophysiology of clinical manifestations still lacks knowledge. The objective of this paper was to evaluate metabolite abundance in the saliva of long COVID patients 60 days after hospital discharge.

Immune landscape of the tumour microenvironment in Ethiopian breast cancer patients.

Background: The clinical management of breast cancer (BC) is mainly based on the assessment of receptor expression by tumour cells. However, there is still an unmet need for novel biomarkers important for prognosis and therapy. The tumour immune microenvironment (TIME) is thought to play a key role in prognosis and therapy selection, therefore this study aimed to describe the TIME in Ethiopian BC patients.

Acoustic Modulation of Excitonic Complexes in hBN/WSe/hBN Heterostructures.

The interaction of high-frequency surface acoustic waves (SAWs) and excitons in van der Waals heterostructures (vdWHs) offers challenging opportunities to explore novel quantum effects and functionalities. We probe the interaction of neutral excitons, trions, and biexcitons with SAWs in a hBN/WSe/hBN vdWH. We show that neutral excitons respond weakly to the SAW stimulus at 5 K.

Convergent Evolution Associated with the Loss of Developmental Diapause May Promote Extended Lifespan in Bees.

Diapause has long been proposed to play a significant role in the evolution of eusociality in Hymenoptera. Recent studies have shown that shifts in the diapause stage precede social evolution in wasps and bees; however, the genomic basis remains unknown. Given the overlap in molecular pathways that regulate diapause and lifespan, we hypothesized that the evolutionary loss of developmental diapause may lead to extended lifespan among adults, which is a prerequisite for the evolution of eusociality.

Asian American Representation in Medicine by Career Stage and Residency Specialty.

Importance: Asian American individuals are not underrepresented in medicine; however, aggregation in prior workforce analyses may mask underlying disparities.

Objective: To assess representation by Asian race and disaggregated subgroups in the US allopathic medical school workforce.

Design, Setting, And Participants: This cross-sectional study used Association of American Medical Colleges (AAMC) special reports, generated using the AAMC Applicant-Matriculant Data File, Student Records System, Graduate Medical Education Track Survey, and faculty roster.

Enzyme Benchmarking with Polyethylene Furanoate Soluble Scaffolds for Directed Evolution of PEFases.

Plastic waste is a major threat in our industrialized world and is driving research into bioplastics. The success of biobased polyethylene furanoate (PEF) as a viable alternative to polyethylene terephthalate (PET) of fossil origin will depend on designing effective enzymes to break it down, aiding its recycling. Here, a panel of fungal and bacterial cutinases were functionally expressed in a tandem yeast expression system based on and .

Phytochemical Profiling by HPLC-ESI-MS/MS and In Vitro Investigation of the Antidiabetic Activity of Cassia bakeriana Bark Extract and Fractions.

Type 2 diabetes mellitus is a global health problem, placing patients at a higher risk of developing cardiovascular diseases and cancer. This study investigates the antidiabetic potential of Cassia bakeriana bark extracts and fractions. We evaluate their ability to inhibit α-amylase and α-glucosidase enzymes and advanced glycation end-products (AGEs).