Role of serum matrix metalloproteinase in the diagnosis of gastric cancer.

Objective: To determine the clinical value of a matrix metalloproteinase (MMP) antibody array in diagnosing gastric cancer (GC).

Methods: In this prospective study, serum samples of patients with GC (n=66) and non-neoplastic gastric disease (NGD; n=34) were collected between November 2017 and July 2018. The quantitative measurement of 10 MMP-related proteins was done using MMP arrays and compared between the two groups.

Free amino acid profiling of gastric juice as a method for discovering potential biomarkers of early gastric cancer.

Prior studies showed that aromatic amino acids (AAAs) could be used as potential gastric juice biomarkers in screening gastric cancer (GC). To identify new biomarkers for early diagnosis of GC, the characteristics of gastric juice free amino acid (GJFAA) profiling was determined. First, gastric juice was collected from 130 consecutive patients who underwent gastroscopy.

A randomized, double blind, controlled, multi center study of Ilaparazole in the treatment of reflux esophagitis-Phase III clinical trial.

Aim And Background: Proton pump inhibitors (PPIs) are the main drugs for the treatment of reflux esophagitis. Phase II clinical trials showed that, compared with Esomeprazole, the new PPI Ilaparazole is great in terms of efficacy for reflux symptoms relief and curling for esophagitis. The aim of this study was to confirm suitable dose of Ilaparazole in the treatment of reflux esophagitis.

Metabolic profiles of gastric cancer cell lines with different degrees of differentiation.

Gastric cancer is a commonly found malignant tumor, yet research on biomarkers of gastric cancer still face tremendous challenges. This study is the first to use gas chromatography-mass spectrometry (GC-MS) to measure and compare the metabolic profiles of gastric cancer cell lines with varying degrees of differentiation (MKN-28, SGC-7901, and AGS) with that of a normal gastric epithelial cell line (GES-1). OPLS-DA models were established to distinguish gastric cancer cell lines from a normal gastric epithelial cell line.

Non- (NHPHs) Induce Shifts in Gastric Microbiota in -Infected Patients.

To explore the effects of gastric non- species(NHPH) on the structure and potential function of gastric microbiota, we employed 16S rRNA gene sequencing on 164 gastric biopsy specimens from NHPH () / coinfection individuals, monoinfection individuals and healthy controls. The results demonstrated that marked structural and functional variations between mono- and coinfection samples (HPHS, HPHF, HPHM). The changes in bacterial structure induced by NHPH are mainly attributed to their ability of gastric acid secretion inhibition as well as bacterial chemotaxis.

Gastric Juice-Based Real-Time PCR for Tailored Treatment: A Practical Approach.

A gastric juice-based real-time polymerase chain reaction (PCR) assay was established to identify infection, clarithromycin susceptibility and human genotypes and to guide the choice of proton pump inhibitor (PPI), clarithromycin and amoxicillin treatment for tailored eradication therapy. From January 2013 to November 2014, 178 consecutive dyspeptic patients were enrolled for collection of gastric biopsy samples and gastric juice by endoscopy at the Peking University Third Hospital; 105 and 73 -positive and -negative patients, respectively, were included in this study. infection was defined as samples with both a strongly positive rapid urease test (RUT) and positive histology.

A Randomized, Double-blind, Active-Controlled, Multi-center Study of Ilaprazole in the Treatment of Reflux Esophagitis.

Background And Objective: Proton pump inhibitors (PPIs) are the main drugs for the treatment of reflux esophagitis. Previous studies have indicated ilaprazole to be safer and more effective in treating duodenal ulcers as compared with omeprazole. Being a novel PPI, ilaprazole may be used in the treatment of reflux esophagitis.

Tailored versus Triple plus Bismuth or Concomitant Therapy as Initial Helicobacter pylori Treatment: A Randomized Trial.

Background: With markedly increased antibiotic resistance and unsatisfactory efficacies of common empiric eradication regimens in the mainland of China, tailored therapy may be the best choice to achieve good efficacy. This study compared the eradication rates, safety, and compliance of tailored therapy to those of triple therapy plus bismuth and concomitant therapy in the naïve patients with Helicobacter pylori infection.

Materials And Methods: Between September 2013 and April 2014, 1050 patients with H.

Effect of Helicobacter pylori eradication on reflux esophagitis therapy: a multi-center randomized control study.

Background: Helicobacter pylori (H. pylori) frequently colonizes the stomach. Gastroesophageal reflux disease (GERD) is a common and costly disease.

Therapeutic effect of esophageal foreign body extraction management: flexible versus rigid endoscopy in 216 adults of Beijing.

Background: The aim of this study was to assess the effectiveness and complications of rigid endoscopy (RE) and flexible endoscopy (FE) for the extraction of esophageal foreign bodies (FB) in adults.

Material And Methods: A retrospective analysis was conducted on the medical records of 216 adult patients with esophageal FB impaction treated at Peking University Third Hospital, Beijing, China, between January 2008 and December 2012.

Results: The success rate of FB extraction was 100% (142/142) in patients treated with RE compared to 97.

[The clinical significance of typical reflux symptoms in diagnosing gastroesophageal reflux disease].

Objective: To explore the clinical significance of typical reflux symptoms in the diagnosis of gastroesophageal reflux disease (GERD).

Methods: Consecutive patients older than 16 years, who initially visited department of gastroenterology at clinic of Peking University Third Hospital from May 9, 2012 to Dec 31, 2012, were required to complete a self-reported GERD questionnaire. Upper endoscopy was performed in some selected patients.

Relationship of Helicobacter pylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study.

Background: Helicobacter pylori (Hp) is a common and potentially curable cause of gastric mucosa lesion. This study investigated the relationship of Hp infection with histological changes in gastric mucosa and gastric cancer in Hp-positive patients compared with Hp-eradication patients followed up for ten years.

Methods: From an initial group of 1 006 adults, 552 Hp-positive subjects were randomly assigned to a treatment group (T; n = 276) or a placebo group (P; n = 276).

MicroRNA-21 stimulates gastric cancer growth and invasion by inhibiting the tumor suppressor effects of programmed cell death protein 4 and phosphatase and tensin homolog.

Purpose: MicroRNA-21 (miR-21) is abnormally expressed in many solid cancers, such as gastric adenocarcinoma, and regulates some targets involved in cancer initiation and progression. In this study, we investigated the function of miR-21 in two gastric cancer cell lines, as well as its potential targeting of the tumor suppressor genes phosphatase and tensin homolog (PTEN) and programmed cell death protein 4 (PDCD4).

Methods: The first step was to use quantitative (q) RTPCR in order to verify the overexpression of miR-21 in two different gastric cancer cell lines (SGC-7901 and MKN-45) transfected with mIR-21 mimic.

A comparative study of sequential therapy and standard triple therapy for Helicobacter pylori infection: a randomized multicenter trial.

Objectives: Studies conducted in large populations of patients and providing full information on Helicobacter pylori (H. pylori) antibiotic resistance are needed to determine the efficacy of sequential therapy (SQT) against this pathogen. This study compared eradication rates with SQT and standard triple therapy (STT), and evaluated the impact of antibiotic resistance on outcomes.

[Analysis for 171 cases of esophageal foreign bodies impacted in upper esophagus with endoscopic treatment].

Objective: To assess the effectiveness and complications of rigid endoscopy (RE) and flexible endoscopy (FE) for the extraction of esophageal foreign bodies (FBs) in adults.

Methods: A retrospective analysis was conducted on the medical records of 171 adult patients with the upper esophageal FB impaction treated at Peking University Third Hospital, Beijing, China, between January 2008 and December 2012.

Results: In the study, 126 patients with the upper esophageal foreign body impaction were treated with RE, while 45 patients received FE.

Chronic gastritis in China: a national multi-center survey.

Background: Chronic gastritis is one of the most common findings at upper endoscopy in the general population, and chronic atrophic gastritis is epidemiologically associated with the occurrence of gastric cancer. However, the current status of diagnosis and treatment of chronic gastritis in China is unclear.

Methods: A multi-center national study was performed; all patients who underwent diagnostic upper endoscopy for evaluation of gastrointestinal symptoms from 33 centers were enrolled.

[Complication related factors and efficacy with flexible endoscopy of 101 esophageal foreign bodies].

Objective: To analyze the related factors of complications and treatment efficacy with flexible endoscopy for esophageal foreign body (FB).

Methods: In a retrospective study with consecutive data, 101 adults including 52 males and 49 females with esophageal FB impaction between January 2005 and December 2012 admitted into Department of Gastroenterology's Endoscopic Unit at Peking University Third Hospital were included, aged (49 ± 21) years.

Results: (1) FB impaction in upper and middle esophagus accounted for 87.

High levels of aromatic amino acids in gastric juice during the early stages of gastric cancer progression.

Background: Early-stage gastric cancer is mostly asymptomatic and can easily be missed easily by conventional gastroscopy. Currently, there are no useful biomarkers for the early detection of gastric cancer, and their identification of biomarkers is urgently needed.

Methods: Gastric juice was obtained from 185 subjects that were divided into three groups: non-neoplastic gastric disease (NGD), advanced gastric cancer and early gastric cancer (EGC).

[The endoscopic and clinical features of Indigo Naturalis-associated ischemic lesions of colonic mucosa].

Objective: By analysing the clinical features of Indigo Naturalis-associated ischemic lesion of colon mucosa to improve the precautionary and therapeutic level of the disease.

Methods: Thirteen patients diagnosed as Indigo Naturalis-associated ischemic lesion of colon mucosa in Peking University Third Hospital from 2005 to 2010 were reviewed. The endoscopic and clinical features were analysed.

Ilaprazole for the treatment of duodenal ulcer: a randomized, double-blind and controlled phase III trial.

Objective: The new proton pump inhibitor (PPI), ilaprazole performed better at the dose of 10 mg/d relative to 5 or 20 mg/d in a previous phase II trial. A larger phase III trial was carried out to confirm the efficacy and safety of ilaprazole (10 mg/d) compared with omeprazole (20 mg/d) and provide some characteristics of the relationship between ilaprazole metabolism and CYP2C19 for later studies.

Research Design And Methods: Patients with at least one endoscopically diagnosed active duodenal ulcer (DU) were enrolled in a multicenter, randomized, double-blind, positive controlled trial and then assigned randomly to the ilaprazole group (10 mg/d) or the omeprazole group (20 mg/d) with a sample allocation ratio 2:1.

Three aromatic amino acids in gastric juice as potential biomarkers for gastric malignancies.

For screening early-stage gastric malignancies, the existing serum biomarkers have limited sensitivity and specificity. Gastric juice biomarkers are scarce and require further investigation. We divided this study on searching potential biomarkers into four parts: (1) detection of differential fluorescence spectrum and peaks in the gastric juice from patients using fluorescence spectroscopy and HPLC, (2) identification and validation of differential peaks using LC/MS and NMR, (3) quantification of potential biomarkers, and (4) establishment of diagnostic detection.