Publications by authors named "Sanna Junttila"
Article Synopsis
- - The study focuses on using 3D organoids, specifically renal organoids, to better model and understand kidney cancer by comparing gene profiles from embryonic mouse tissue and tumor biopsies from kidney cancer patients.
- - Researchers identified specific genes related to kidney development and cancer that affect cell migration and viability when silenced, using siRNA techniques on renal cell carcinoma (RCC) cells.
- - Findings suggest that combining kidney tissue with cancer cells in 3D organoids helps reveal how cancer cells impact kidney tissue development, indicating potential for new gene screens related to kidney cancer and improved modeling of tumor behaviors.
The kidney vasculature is critical for renal function, but its developmental assembly mechanisms remain poorly understood and models for studying its assembly dynamics are limited. Here, we tested whether the embryonic kidney contains endothelial cells (ECs) that are heterogeneous with respect to VEGFR2/Flk1/KDR, CD31/PECAM, and CD146/MCAM markers. Tie1Cre;R26R(YFP)-based fate mapping with a time-lapse in embryonic kidney organ culture successfully depicted the dynamics of kidney vasculature development and the correlation of the process with the CD31(+) EC network.
View Article and Find Full Text PDFThe embryonic mammalian metanephric mesenchyme (MM) is a unique tissue because it is competent to generate the nephrons in response to Wnt signaling. An ex vivo culture in which the MM is separated from the ureteric bud (UB), the natural inducer, can be used as a classic tubule induction model for studying nephrogenesis. However, technological restrictions currently prevent using this model to study the molecular genetic details before or during tubule induction.
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