Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay.

Introduction: Streptococcus pyogenes (group A Streptococcus, GAS) is responsible for a variety of highly communicable infections, accounting for 5-15 and 20-30% of sore throat hospital visits in adults and children, respectively. Prompt diagnosis of GAS can improve the quality of patient care and minimize the unnecessary use of antibiotics.

Objective: Our objective was to develop an alternative nucleic acid amplification method for the diagnosis of GAS.