DNA Barcoding of Invasive Terrestrial Plant Species in India.

Invasive plants are known to cause biodiversity loss and pose a major risk to human health and environment. Identification of invasive plants and distinguishing them from native species has been relied on morphological examination. Stringent requirement of floral characters and decreasing number of expert taxonomists are making conventional morphology-based identification system tedious and resource-intensive.

Optical detection of total cholesterol based on a dye-displacement method.

In the present study, the dye methylene blue (MB) was entrapped in an agarose gel and used as a sensing probe for the detection of total cholesterol. When methylene blue-entrapped agarose cubes were added to the cholesterol solution, methylene blue was displaced by cholesterol and released into the solution. A calibration curve was prepared by plotting the rate of release of methylene blue at 664 nm against varying cholesterol concentrations.

Revisiting DNA barcoding of true bugs of the infraorder Pentatomomorpha (Hemiptera: Heteroptera) from India.

Cytochrome c oxidase subunit I gene (COI) sequences of roughly 509 bp length for various species of the Infraorder Pentatomomorpha were generated. K2P divergences within and between species and genera were calculated and compared using newly generated sequences and the ones available on online portals. Mean interspecific (within-genus) genetic divergence (14.

DNA barcoding of Pentatomomorpha bugs (Hemiptera: Heteroptera) from Western Ghats of India.

Recent studies from East Asia and Canadian National Collection of Insects have established the utility of DNA barcoding technique in identification of true bugs. The present study is an expansion of the database by adding mitochondrial cytochrome c oxidase I (mtCOI) sequences from forty three species of indigenous true bugs of India. mtCOI gene analysis of infraorder Pentatomomorpha covering a total of seventy three species that belong to five superfamilies; Pentatomoidea, Coreoidea, Pyrrhocoroidea, Lygaeoidea and Aradoidea revealed more than 3% interspecific distances in all the taxa studied except for two cases which showed barcode sharing.

Glutaraldehyde activated eggshell membrane for immobilization of tyrosinase from Amorphophallus companulatus: application in construction of electrochemical biosensor for dopamine.

Tyrosinase from a plant source Amorphophallus companulatus was immobilized on eggshell membrane using glutaraldehyde. Among the three different approaches used for immobilization, activation of eggshell membrane by glutaraldehyde followed by enzyme adsorption on activated support could stabilize the enzyme tyrosinase and was found to be effective. K(m) and V(max) values for dopamine hydrochloride calculated from Lineweaver-Burk plot were 0.

Electrochemical biosensor for catechol using agarose-guar gum entrapped tyrosinase.

An electrochemical biosensor using tyrosinase was constructed for the determination of catechol. The enzyme was extracted from a plant source Amorphophallus companulatus and entrapped in agarose-guar gum composite biopolymer matrix. Catechol was determined by direct reduction of biocatalytically liberated quinone species at -0.

Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film.

An electrochemical enzyme electrode for dopa and dopamine was developed via an easy and effective immobilization method. The enzyme tyrosinase was extracted from a plant source Amorphophallus companulatus and immobilized in a novel composite of two biopolymers: agarose and guar gum. This composite matrix-containing enzyme forms a self-adhering layer on the active surface of glassy carbon electrode, making it a selective and sensitive phenol sensor.