Publications by authors named "Sanjeet Kumar"

Article Synopsis
  • Emergence of carbapenem-resistant A. baumannii (CRAB) poses a significant global healthcare threat, necessitating studies of both CRAB and its susceptible counterpart, A. baumannii (CSAB).
  • The study reveals distinct evolutionary pathways for CRAB and CSAB, with CRAB primarily represented by a successful clone (ST2) forming a specific phylogroup (PG-I), while CSAB isolates display greater diversity.
  • Insights from genomic analysis highlight the role of recombination and genome plasticity in the development of drug resistance, which is crucial for managing emerging drug-resistant pathogens in hospitals.
View Article and Find Full Text PDF

is a versatile bacterium found in plants, water, air, and even hospital settings. Deep taxono phylogenomics studies have revealed that is a complex of several hidden species that are not differentiated using conventional approaches. In the last two decades, there have been increasing reports of as a pathogen of diverse plants.

View Article and Find Full Text PDF
Article Synopsis
  • CC31 clonal complex, a major cause of infections in non-cystic fibrosis patients in India, exhibits significant antibiotic resistance and virulence, necessitating better understanding for improved treatment strategies.* -
  • Analysis of 35 CC31 isolates revealed 11 unique sequence types, with 5 new ones exclusively from India, indicating distinct evolutionary paths compared to global cystic fibrosis isolates.* -
  • Resistance patterns showed 100% of isolates had genes for tetracyclines and aminoglycosides, with notable resistance to chloramphenicol and levofloxacin, raising concerns about the effectiveness of current treatment options.*
View Article and Find Full Text PDF

The advent of high-throughput sequencing and population genomics has enabled researchers to investigate selection pressure at hypervariable genomic loci encoding pathogen-associated molecular pattern (PAMP) molecules like lipopolysaccharide (LPS). Xanthomonas is a model and a major group of phytopathogenic bacteria that infect hosts in tissue-specific manner. Our in-depth population-based genomic investigation revealed the emergence of major lineages in two Xanthomonas pathogens that infect xylem of rice and sugarcane is associated with the acquisition and later large-scale replacement by distinct type of LPS cassettes.

View Article and Find Full Text PDF
Article Synopsis
  • Bacillus licheniformis MCC 2514 is recognized as a promising probiotic, with whole genome analysis revealing crucial molecular factors contributing to its probiotic potential, including a genome of 4,230,480 bp and genes involved in biocontrol properties.
  • The study identified a pangenome of 6008 genes and core genome of 3775 genes, which included clusters for producing beneficial compounds like NRPS and bacteriocins, as well as genes for carbohydrate degradation, stress tolerance, and gut adhesion.
  • Furthermore, MCC 2514 demonstrated its effectiveness in food preservation by improving the shelf life of idli batter, reducing coliform contamination, and maintaining suitable pH levels, showcasing its bio-preservative capabilities.
View Article and Find Full Text PDF

The genus Streptococcus, a member of family Streptococcaceae, is known for its wide range of industrial, clinical and human relevance. Among the species of genus Streptococcus two members, namely Streptococcus koreensis and Streptococcus ilei, were isolated from subgingival dental plaque and human small intestinal fluid, respectively. The 16S rRNA gene sequence similarity of the type strains of these members shows a similarity of 99.

View Article and Find Full Text PDF
Article Synopsis
  • The systematic analysis using different genomic methods indicates very high genome similarity percentages, suggesting they belong to the same species, with values exceeding established species delineation cutoffs.
  • The phylogenetic analyses reinforce that these strains are monophyletic, leading to the proposal that S. ursoris should be reclassified as a synonym of S. ratti.
View Article and Find Full Text PDF
Article Synopsis
  • The genus Leuconostoc includes various lactic acid bacteria important for dairy and food industries, but its taxonomy is outdated and relies on classical methods.
  • Recent studies reveal that a genome-based analysis identified sixteen species, including three new ones, and highlighted significant reshuffling among known species such as L. mesenteroides and L. lactis.
  • The presence of antibiotic resistance genes in these beneficial bacteria raises concerns, and a comprehensive genome study could improve taxonomy and enhance understanding of their biotechnological applications.
View Article and Find Full Text PDF
Article Synopsis
  • Genus is a newly identified group of gamma-proteobacterium found in various environments, particularly in hydrocarbon-polluted areas.
  • The morphology of species in this genus is similar to certain other strains, but their genome has not been thoroughly studied.
  • This research provides the complete genome sequences of the type strains of Genus to compare and understand their differences from related genera.
View Article and Find Full Text PDF

Background: Emergence of new variant of SARS-CoV-2, namely omicron, has posed a global concern because of its high rate of transmissibility and mutations in its genome. Researchers worldwide are trying to understand the evolution and emergence of such variants to understand the mutational cascade events.

Methods: We have considered all omicron genomes (n = 302 genomes) available till 2nd December 2021 in the public repository of GISAID along with representatives of variants of concern (VOC), i.

View Article and Find Full Text PDF

Based on phylo-taxonogenomics criteria, we present amended descriptions for 20 pathovars to . Incidentally, 18 were first reported from India. Seven out of twenty are classified as , 12 out of 20 as , and one as .

View Article and Find Full Text PDF

Background: COVID-19 has posed unforeseen circumstances and throttled major economies worldwide. India has witnessed two waves affecting around 31 million people representing 16% of the cases globally. To date, the epidemic waves have not been comprehensively investigated to understand pandemic progress in India.

View Article and Find Full Text PDF
Article Synopsis
  • Genus Xanthomonas includes various plant pathogens related to Xylella, Stenotrophomonas, and Pseudoxanthomonas, which display diverse lifestyles and genetic traits.
  • Xylella fastidiosa, a severe plant pathogen, has a significantly reduced genome and is primarily transmitted by insects to its host plants, causing wilting in crops like grapes and citrus.
  • The study highlights the importance of integrating classical phenotypic data with genomic insights to better understand the taxonomic relationships between Xanthomonas and its related genera, noting that Xylella is a minor variant within the Xanthomonas lineage.
View Article and Find Full Text PDF

We report three yellow-pigmented, Gram-negative, aerobic, rod-shaped, motile bacterial isolates designated as PPL1, PPL2, and PPL3 from healthy basmati rice seeds. Phenotypic and 16S rRNA gene sequence analysis assigned these isolates to the genus Xanthomonas. The 16S rRNA showed a 99.

View Article and Find Full Text PDF

is a typical example of an environmental originated opportunistic human pathogen, which can thrive at different habitats including the human body and can cause a wide range of infections. It must cope with heat stress during transition from the environment to the human body as the physiological temperature of the human body (37 °C) is higher than environmental niches (22–30 °C). Interestingly, a phylogenetic neighbour of within genus is unable to grow at 37 °C.

View Article and Find Full Text PDF

Background: Night-soil compost (NSC) has traditionally been conserving water and a source of organic manure in northwestern Himalaya. Lately, this traditional method is declining due to modernization, its unhygienic conditions, and social apprehensions. Reduction in the age-old traditional practice has led to excessive chemical fertilizers and water shortage in the eco-sensitive region.

View Article and Find Full Text PDF

The medicinal herb, Picrorhiza kurroa Royle ex Benth has become endangered because of indiscriminate over-harvesting. Although micropropagation has been attempted for mass propagation of the plant, survival of in vitro plantlets under green house/open field poses a major challenge. Biopriming of micropropagated plantlets with plant growth-promoting rhizobacteria (PGPR) are among the successful methods to combat this problem.

View Article and Find Full Text PDF

pv. () is a serious pathogen causing bacterial blight disease in rice. Population genomic studies have revealed that rampant inter-strain rather than inter-lineage differences are contributing to the evolutionary success of this pathogen.

View Article and Find Full Text PDF
Article Synopsis
  • - The study presents the first complete genomic analysis of a drug-resistant strain of Stenotrophomonas maltophilia from a hospital in India, which shows resistance to common antibiotics like trimethoprim/sulfamethoxazole and levofloxacin.
  • - It identifies unique genomic features, including fourteen dynamic regions and two super-integrons containing multiple antibiotic and disinfectant resistance genes, highlighting the strain's adaptability in a hospital environment with high antimicrobial use.
  • - The research emphasizes the need for advanced genomic techniques to monitor and understand the spread of extensively drug-resistant pathogens, which pose a significant threat to global healthcare systems, especially in countries with high patient loads.
View Article and Find Full Text PDF

species are primarily known as a group of phytopathogens infecting diverse plants. Recent molecular studies reveal the existence of potential novel species and strains of following a nonpathogenic lifestyle. In the present study, we report whole-genome sequences of four nonpathogenic strains from citrus (NPXc).

View Article and Find Full Text PDF

Order (earlier known ) is a taxonomically complex group of a large number of gamma-proteobacteria classified in two different families, namely and . Current taxonomy is largely based on classical approaches and is devoid of whole-genome information-based analysis. In the present study, we have taken all classified and poorly described species belonging to the order to perform a phylogenetic analysis based on the 16 S rRNA sequence.

View Article and Find Full Text PDF

Male sterility is of high importance in hybrid seed production of hot and sweet peppers. Genic (or nuclear) male sterility (GMS) is a simply inherited (usually monogenic recessive) and highly stable trait. However, one major disadvantage of using GMS is 1:1 segregation of male sterile to male fertile plants in every subsequent generation.

View Article and Find Full Text PDF