Development of RT-LAMP assay for detection of lead and cadmium toxicity using HepG2 cells.

Heavy metals such as lead and cadmium are prevalent in the environment. These are harmful to living beings even at lower concentrations as they persist in the body for years and lead to the development of severe diseases. Therefore, the present work was designed to develop a rapid and field-applicable cell-based assay for quick detection of lead and cadmium in biofluids using an RNA biomarker.

Insights on therapeutic approaches of natural anti-Alzheimer's agents in the management of Alzheimer's disease: A future perspective.

In the current scenario, Alzheimer's disease is a complex, challenging, and arduous health issue, and its prevalence, together with comorbidities, is accelerating around the universe. Alzheimer's disease is becoming a primary concern that significantly impacts an individual's status in life. The traditional treatment of Alzheimer's disease includes some synthetic drugs, which have numerous dangerous side effects, a high risk of recurrence, lower bioavailability, and limited treatment.

Comprehensive proteomic analysis of buffalo milk extracellular vesicles.

Extracellular vesicles are secretory vesicles involved in cell-to-cell communication via their encapsulated cargo of proteins, lipids, and nucleic acids. Bovine milk provides a rich source of extracellular vesicles (mEVs) that have been studied as therapeutics and drug delivery systems. Therefore, insight into the mEV cargo, such as its proteome, may help in understanding the molecular mechanism underlying the potential health benefits attributed to the mEVs.

Effect of Electron-Donating Substituents and an Electric Field on the Δ of Selected Imidazopyridine Derivatives: A DFT Study.

A series of thermally activated delayed fluorescent (TADF) molecules having an imidazopyridine acceptor, a benzene linker, and a 9,9-dimethyl-9,10-dihydroacridine donor are designed and examined using a quantum chemical approach. The above framework spatially separates the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO), minimizing their overlap, ultimately resulting in a reduced energy gap between the excited singlet and triplet states (Δ). The impact of electron-donating substituents (-Me, -Et, --Bu, -OMe, and -NMe) on the donor moiety of the parent molecule 2-(4-(9,9-dimethylacridin-10(9H)-yl)phenyl)imidazo[1,2-]pyridine-3,6-dicarbonitrile (Ac-CNImPy) is investigated.

The Anticancer Journey of Liquiritin: Insights into Its Mechanisms and Therapeutic Prospects.

Liquiritin (LIQ), a bioactive flavonoid from Glycyrrhiza species, has shown significant potential in cancer therapy. LIQ exhibits potent inhibitory effects on various cancer cell types, including breast, lung, liver, and colon cancers, while demonstrating low toxicity towards healthy cells. Its anticancer mechanisms include inducing cell cycle arrest, promoting apoptosis, and modulating inflammation-related pathways.

Escaping a Nightmare: Successfully Retrieving a Fractured Guidewire During Percutaneous Coronary Intervention.

A guidewire fracture seldom occurs as a complication of percutaneous coronary intervention (PCI). Guidewire fragments retained in the coronary tree can result in thrombosis, embolic phenomena, dissection, perforation, and vessel occlusion. This study represents a rare incidence of fractured guide wire, which occurred during PCI in a 44-year-old male due to the acute angle and heavy calcification which was safely and successfully retrieved using a 4×40 mm Solitaire device (Irvine, CA: Medtronic) (neurovascular remodeling device).

Global longitudinal active strain energy density (GLASED): age and sex differences between young and veteran athletes.

Background: Global longitudinal active strain energy density (GLASED) is an innovative method for assessing myocardial function and quantifies the work performed per unit volume of the left ventricular myocardium. The GLASED, measured using MRI, is the best prognostic marker currently available. This study aimed to evaluate the feasibility of measuring the GLASED using echocardiography and to investigate potential differences in the GLASED among athletes based on age and sex.

Quality by design-based development and in vitro evaluation of dual release tablet of etoricoxib and thiocolchicoside: A novel chronotherapeutic approach for arthritis pain management.

Objective: The traditional drug delivery system is not much effective when treating chronopathological diseases like arthritis. Consequently, there is a gap in the market for a delivery system that can provide an explicit treatment following the chronopharmacology of this disorder. The present study is based on the objective to develop Eudragit coated dual release bilayer tablet designed by the quality by design (QbD) and based on the chronotherapeutic approach.

Rapid, Simple and Low-Cost Analytical Method Development for Quantification of Eltrombopag Olamine in Tablet Dosage by UV Spectroscopy Method.

Background: Eltrombopag Olamine is a drug used to treat thrombocytopenia, a disorder where blood platelet counts get lower and severe aplastic anemia. It serves as a thrombopoietin receptor agonist, which give rise to platelet production in the bone marrow.

Objectives: The objective of this study is to develop a simple, specific, accurate, precise and economical Ultraviolet spectroscopy method to estimate the amount of Eltrombopag Olamine in bulk and tablet dosage form.

Air Quality and Public Health Risk Assessment: A Case of an Industrial Area in Haridwar City, Uttarakhand (India).

Background: Air pollution is a significant issue for a developing country like India and the air quality index (AQI) forecasting helps to predict air quality levels in advance and allows individuals to take precautionary measures to protect their health.

Objectives: The study aimed to forecast the AQI for an industrial area (SIDCUL, Haridwar City) using a time series regression model.

Materials And Methods: Three years of existing AQI data points (post-COVID-19) were collected from the Uttarakhand Pollution Control Board for the SIDCUL area of Haridwar City and tried to know the status of AQI values for the following 12 months.

Small interfering RNA (siRNA) as a potential gene silencing strategy for diabetes and associated complications: challenges and future perspectives.

Objective: This article critically reviews the recent search on the use of Small Interfering RNA (siRNA) in the process of gene regulation that has been harnessed to silence specific genes in various cell types, including those involved in diabetes complications.

Significance: Diabetes, a prevalent and severe condition, poses life-threatening risks due to elevated blood glucose levels. It results from inadequate insulin production by the pancreas or ineffective insulin utilization by the body.

A hybrid SWAT-ANN model approach for analysis of climate change impacts on sediment yield in an Eastern Himalayan sub-watershed of Brahmaputra.

The current study focuses on analyzing the impacts of climate change and land use/land cover (LULC) changes on sediment yield in the Puthimari basin, an Eastern Himalayan sub-watershed of the Brahmaputra, using a hybrid SWAT-ANN model approach. The analysis was meticulously segmented into three distinct time spans: 2025-2049, 2050-2074, and 2075-2099. This innovative method integrates insights from multiple climate models under two Representative Concentration Pathways (RCP4.

Comparative study of antioxidant activities of Allium sativum (a novel variety, HG17) and Allium ampeloprasum (SMG): Revealing the higher potential of HG17 and analyzing its phytochemicals.

Garlic, belonging to the genus Allium, is renowned for its rich antioxidant potential. Snow Mountain garlic (SMG) (Allium ampeloprasum) has been traditionally used for medicinal purposes because of its higher antioxidant potential. Considering its potential in medical therapies, we compared the antioxidant activity of SMG with a novel variety of Allium sativum, Hisar garlic 17 (HG17).

International Criteria for Reporting Study Quality for Sudden Cardiac Arrest/Death Tool.

Background: Studies reporting on the incidence of sudden cardiac arrest and/or death (SCA/D) in athletes commonly lack methodological and reporting rigor, which has implications for screening and preventative policy in sport. To date, there are no tools designed for assessing study quality in studies investigating the incidence of SCA/D in athletes.

Methods And Results: The International Criteria for Reporting Study Quality for Sudden Cardiac Arrest/Death tool (IQ-SCA/D) was developed following a Delphi process.