Publications by authors named "Sanjay Mane"
Article Synopsis
- This study introduces a microfluidic device designed to efficiently separate white blood cells (WBCs) from red blood cells (RBCs) for the Nitroblue Tetrazolium (NBT) bioassay, which measures phagocytic activity in cells.
- The device successfully removes 99.99% of RBCs from WBC samples, improving the RBC to WBC ratio from 848:1 to 2:3, and is effective even at a hematocrit concentration of 1% blood.
- The microfluidic device is easy to fabricate, cost-effective, and can process a single drop of blood in under 10 minutes, without the need for complex methods like RBC lysis or expensive
White blood cells (WBCs) are robust defenders during antigenic challenges and prime immune cell functioning indicators. High-purity WBC separation is vital for various clinical assays and disease diagnosis. Red blood cells (RBCs) are a major hindrance in WBC separation, constituting 1000 times the WBC population.
View Article and Find Full Text PDFThere is a possibility of in-situ physicochemical interactions between concomitantly administered drugs. This study aimed to investigate such physicochemical interactions between pioglitazone and rifampicin. Pioglitazone exhibited significantly higher dissolution in the presence of rifampicin, while the dissolution of rifampicin remained unaffected.
View Article and Find Full Text PDFUnlabelled: Microfluidic methods act as an effective motile sperm separation technique used in infertility treatments. This work presents a standalone microfluidic device to separate motile sperm cells from non-motile sperm cells and debris. The separation mechanism is based on the centrifugal force acting on sperms and the ability of progressive motile sperms to swim upstream.
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