Publications by authors named "Sanja Vanhatalo"
Objective: Appendicoliths are associated with a more complicated course of acute appendicitis and failure of non-operative treatment. We aimed to update the appendicolith classification originally described in 1966 and to assess the association of appendicolith characteristics with appendicitis severity.
Design: This prospective predefined MAPPAC-trial (ClinicalTrials.
Article Synopsis
- The study focuses on improving DNA isolation methods for large-scale microbiome research by testing different preservatives and pre-treatment protocols on fecal samples to ensure high-quality DNA yields without contamination.
- Various techniques were assessed, including bead beating and proteinase K incubation, with findings indicating that bead beating can enhance bacterial diversity, particularly increasing levels of beneficial bacteria like Bifidobacterium.
- The extraction process utilized a specific kit and incorporated controls for contamination detection, ultimately revealing that sample profiles differ based on the sequencing regions used, which affects the observed bacterial diversity.
Background: Uncomplicated and complicated acute appendicitis seem to be two different forms of this common abdominal emergency. The contribution of appendiceal microbiota to appendicitis pathogenesis has been suggested, but differences between uncomplicated and complicated appendicitis are largely unknown. We compared the appendiceal microbiota in uncomplicated and complicated acute appendicitis.
View Article and Find Full Text PDFIntroduction: Based on the epidemiological and clinical data, acute appendicitis can present either as uncomplicated or complicated. The aetiology of these different appendicitis forms remains unknown. Antibiotic therapy has been shown to be safe, efficient and cost-effective for CT-confirmed uncomplicated acute appendicitis.
View Article and Find Full Text PDFHost genetic factors affecting the gut microbiome play an important role in obesity, yet limited attention has been paid on the host genetic factors linked to physical fitness in modifying the microbiome. This study determined whether sibling-matched pairs of rats selectively bred for high (HCR) and low (LCR) aerobic capacity differ in their microbiome age-dependently and which taxa associate with differential in metabolism. Several taxa in young adult rats (hereafter young) linked to inherited aerobic capacity, while in older adult (hereafter old) rats most of the differences between the lines associated with body weight.
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