Multi-pass, single-molecule nanopore reading of long protein strands.

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for the long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array.

Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity.

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array.