An epitope-tagged Swd2 reveals the different requirements of Swd2 concentration in H3K4 methylation and viability.

Swd2/Cps35 is a common component of the COMPASS H3K4 methyltransferase and CPF transcription termination complex in Saccharomyces cerevisiae. The deletion of SWD2 is lethal, which results from transcription termination defects in snoRNA genes. This study isolated a yeast strain that showed significantly reduced protein level of Swd2 following epitope tagging at its N-terminus (9MYC-SWD2).

Adjusted color probability codes for peripapillary retinal nerve fiber layer thickness in healthy Koreans.

Background: Though a newly developed spectral domain optical coherence tomography (OCT) is at the center of interests for many ophthalmologic researchers and clinicians, its own characteristics are not fully evaluated yet. The main purpose of this study was to establish the adjusted color probability codes for peripapillary retinal nerve fiber layer (RNFL) thickness in healthy Koreans and to compare them with original color codes provided by spectral domain OCT.

Methods: Two hundred ninety-five healthy Korean eyes were enrolled and their peripapillary RNFL thickness was measured by Cirrus OCT.

Spreading-rate dependence of melt extraction at mid-ocean ridges from mantle seismic refraction data.

A variety of observations indicate that mid-ocean ridges produce less crust at spreading rates below 20 mm yr(-1) (refs 1-3), reflecting changes in fundamental ridge processes with decreasing spreading rate. The nature of these changes, however, remains uncertain, with end-member explanations being decreasing shallow melting or incomplete melt extraction, each due to the influence of a thicker thermal lid. Here we present results of a seismic refraction experiment designed to study mid-ocean ridge processes by imaging residual mantle structure.