Publications by authors named "Sang-Gi Jeong"
Optimization of culture conditions is important to improve oocyte maturation and subsequent embryo development. In particular, this study analyzed the effects of increasing concentrations of PIO in the maturation medium on spindle formation and chromosome alignment, glutathione, and intracellular ROS levels and expression of selected genes related to maternal markers, apoptosis, and lipid metabolism. The percentage of oocytes displaying normal spindle formation and chromosome alignment was higher in the 1 µM PIO (1 PIO)-treated group than in the control group.
View Article and Find Full Text PDFThe citrus flavonoid hesperetin has a variety of pharmacological actions, including antioxidant, antiinflammatory, and anticancer activities. This study investigated whether hesperetin prevents aging of oocytes in vitro in which it determined the maturation of nuclear and cytoplasm and the developmental capacity of embryo by modulating the reactive oxygen species (ROS) level. Porcine oocytes were matured in vitro for 44 hr (control) and for an additional 24 hr in the presence of 0, 1, 10, 100, and 250 μM hesperetin (aging, H-1, H-10, H-100, and H-250, respectively).
View Article and Find Full Text PDFSomatic cell nuclear transfer (SCNT) is required for the generation of transgenic animals as disease models. During the in vitro development of SCNT embryos, the quality of matured oocytes is one of the major factors regulating the developmental potential of embryos. Time-lapse monitoring systems are new tools that assess the developmental capacity of embryos for use in embryo transfer.
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- Oxidative stress negatively impacts the quality of porcine oocytes during in vitro maturation (IVM), prompting research on the antioxidant β-cryptoxanthin.
- A concentration of 1µM β-cryptoxanthin significantly improved key maturation indicators, reduced reactive oxygen species, and enhanced antioxidant gene expression in the oocytes compared to the control group.
- Following activation, embryos from the β-cryptoxanthin-treated group showed better blastocyst formation rates, higher cell counts, and increased expression levels of pluripotency and antioxidant genes, suggesting improved developmental potential.
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- Modifying culture media with factors like lysophosphatidic acid (LPA) is crucial for developing oocytes and embryos in vitro.
- This study found that treating porcine embryos with LPA from Days 4 to 7 improved blastocyst formation and embryo size, compared to control embryos.
- LPA treatment also enhanced the expression of specific genes related to cell communication and adhesion, while reducing apoptosis in the embryos.
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- The 0.1 μM AL-treated group showed a higher rate of polar body emission and significantly improved cleavage and blastocyst formation compared to the control group.
- Allicin treatment also altered the expression of certain genes and proteins, suggesting that it enhances oocyte maturation and developmental potential through cellular signaling changes.
Alzheimer's disease (AD) is a progressive neurodegenerative disease associated with memory loss and cognitive impairments. An AD transgenic (Tg) pig model would be useful for preclinical testing of therapeutic agents. We generated an AD Tg pig by somatic cell nuclear transfer (SCNT) using a multi-cistronic vector that harbored three AD-related genes with a total of six well-characterized mutations: hAPP (K670N/M671L, I716V, and V717I), hTau (P301L), and hPS1 (M146V and L286P).
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- The study examines how varying concentrations of Fibroblast Growth Factor 10 (FGF10) affect cumulus cell expansion, oocyte maturation, and embryo development in vitro.
- Results showed that the 10 ng/mL FGF10 treatment significantly improved cumulus cell expansion and resulted in a higher percentage of oocytes with normal spindles compared to controls.
- Additionally, various gene transcripts related to cell functions were upregulated in the FGF10-treated groups, leading to better embryo cleavage rates, blastocyst formation, and overall developmental outcomes.