Comparative Evaluation of Corneal Storage Medias Used as Tooth Avulsion Medias in Maintaining the Viability of Periodontal Ligament Cells Using the Cell Counting Kit-8 Assay.

Purpose: The prime factor in determining the success of reimplantation of an avulsed tooth is the maintenance of the viability of periodontal ligament fibroblast cells (PDFC). This study aims to evaluate and compare Mc Carey Kaufman media (MK), Cornisol, Dulbecco's Modified Eagles Medium (DMEM), Hanks Balanced Salt Solution (HBSS) and distilled water in preserving the viability of the PDFC using the Cell Counting Kit-8 assay (CCK-8).

Methods: Cryopreserved PDFC were suspended in DMEM and incubated in CO incubator at 370C with 95% humidity and 5% CO for attachment.

Anomalies and Clinical Significance of Mylohyoid Nerve: A Review.

Background: The mylohyoid nerve is a branch of the inferior alveolar nerve (IAN), which is a branch of the posterior division of the mandibular nerve (MN). It is the source of motor nerve supply to the mylohyoid and anterior belly of the digastric muscle. At times, it provides sensory innervation to the mandibular teeth and skin below the chin.

Survival of human periodontal ligament fibroblast cells in Cornisol and HBSS for transportation of avulsed teeth: a comparative study.

Objective: Viability of periodontal ligament fibroblast cells (PDFC) is one of the key factors in determining the success of replantation of avulsed teeth. Extra-oral time and transport media are closely related to the same. The present study aims to evaluate and compare the efficiency of Cornisol, Hank's balanced salt solution (HBSS) and normal saline in preserving the viability of PDFC.

Restoration of an endodontically treated premolar with limited interocclusal clearance.

Endodontically treated teeth with the loss of coronal tooth structure when left untreated for a long period may cause supraeruption, drifting, tipping, and rotation of adjacent and opposing teeth. This may be challenging to the clinician, when fabricating a crown because of inadequate interocclusal space. This case report describes a simple technique to restore an endodontically treated maxillary first premolar with the loss of coronal tooth structure and lack of interocclusal space.