Parasitological and clinical-pathological findings in twelve outbreaks of acute trypanosomiasis in dairy cattle in Rio de Janeiro state, Brazil.

In Brazil, infection in cattle was first reported in the state of Pará, in 1944, and the presence of the parasite has already been recorded in several states. The purpose of this study was to report the clinical-pathological aspects of a natural infection by T. vivax in dairy cattle in the state of Rio de Janeiro.

Intelligent monitoring of Aedes aegypti in a rural area of Rio de Janeiro State, Brazil.

The aim of this research was to monitor the presence of females of Aedes aegypti (Linnaeus, 1762) on the Seropédica municipality, Rio de Janeiro State, from 2010 to 2013. For this purpose, the Intelligent Dengue Monitoring (IM-Dengue) and Intelligent Virus Monitoring (IM-Virus) developed by Universidade Federal de Minas Gerais (Ecovec - Minas Gerais, Brazil), were used. IM-Dengue is a tool that allows achieving a weekly overview of A.

Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil.

Background: Anaplasma phagocytophilum is an emerging pathogen of humans, dogs and other animals, and it is transmitted by ixodid ticks. The objective of the current study was a) detect A. phagocytophilum in dogs and ixodid ticks using real-time Polymerase Chain Reaction (qPCR); and b) Determine important variables associated to host, environment and potential tick vectors that are related to the presence of A.

Detection of Anaplasma phagocytophilum in Brazilian dogs by real-time polymerase chain reaction.

Anaplasma phagocytophilum was detected in dogs from Brazil in the municipalities of Seropédica and Itaguaí, Rio de Janeiro state, by real-time polymerase chain reaction (PCR) using SYBR Green to detect the amplification. Of 253 samples, 18 (7.11%) were positive, with a threshold cycle (Ct) ranging from 31 to 35 cycles.

The influence of the fasting period on the number of nymphal instars and on the sex ratio of Argas (Persicargas) miniatus (Acari: Argasidae).

The current study investigated the biology of nymphs of the first and second instars of Argas (Persicargas) miniatus. Nymphs were deprived of food for 15, 30 or 60 days and held at 27 ± 1 °C and 80 ± 10% relative humidity (controlled conditions) or at room conditions of temperature and relative humidity. Nymphs of first instar deprived of food for 15 or 30 days molted to second and third instars in both controlled and room conditions.

Nasopharyngeal intubation in Robin sequence: technique and management.

Objective: To provide a detailed description of the nasopharyngeal intubation (NPI) technique and photographs, which should be helpful for those who may need to perform it for treating the airway obstruction in Robin sequence.

Design: To describe and illustrate the NPI technique and the necessary considerations for its application.

Setting: Hospital de Reabilitação de Anomalias Craniofacial of University of São Paulo, Brazil.