The value of polymerase chain reaction detection of Mycobacterium tuberculosis in granulomas isolated by laser capture microdissection.

Aims: The aim of this study was to investigate the usefulness of polymerase chain reaction (PCR) detection of Mycobacterium tuberculosis in granulomas isolated by laser capture microdissection (LCM).

Methods: The PCR DNA amplification method was used to detect M. tuberculosis in granulomas microdissected from one section stained by haematoxylin and eosin (H&E) from a formalin-fixed paraffin-embedded specimen.

Elastofibroma of the stomach.

We report the case of a 76-year-old woman who presented with abdominal pain caused by a gastric tumor. The stomach was resected and found to have developed an elastofibroma. The lesion was yellowish, unencapsulated, and characterized histologically by abundant collagen fibers mixed with numerous elastic serrated fibers.

[Tuberculosis: a rare and misleading etiology of tongue's ulcer].

We report the case of a 43 year-old man, smoker, who used to live in Africa, consulting for a chronic ulcer of the mobile tongue. An initial biopsy did not show any carcinoma. A second biopsy highlighted an inflammation with numerous tuberculoid granulomas.

[An oral infection not to be underestimated: actinomycosis. A study of 4 cases with extensive bone necrosis].

We report 4 cases of osteitis with extensive necrosis of the maxillofacial bone due to actinomycosis. Histological study showed soft tissue necrosis with bone involvement, suppuration and granulomatous inflammation. At contact with bone destruction, numerous Gram and PAS positive bacteria with branched filaments, inconsistently associated with granules, were noted.

[Langerhans cell histiocytosis of the large bowel].

Gastrointestinal tract involvement in Langerhans'cell histiocytosis (LCH) is unusual. It is most often observed in children, is usually asymptomatic, diagnosed at post mortem examination and associated with a disseminated disease. We report 2 cases of LCH large bowel involvement occuring in a 50-year- old man and in a 71-year-old woman, who presented with digestive symptoms.