Antimicrobial TiN-Ag Coatings in Leather Insole for Diabetic Foot.

This work reports on TiN-Ag antimicrobial coatings deposited by d.c. magnetron sputtering on leather used for insoles on the footwear industry, studies involving the antimicrobial properties of Ag-based functionalized leathers by sputtering techniques are shown.

POTENTIAL CROSS-CONTAMINATION OF SIMILAR Giardia duodenalis ASSEMBLAGE IN CHILDREN AND PET DOGS IN SOUTHERN BRAZIL, AS DETERMINED BY PCR-RFLP.

Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H).

The use of epifluorescence microscopy and fluorescent dyes for visualization of Oxyuris equi eggs.

This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe.

Toxoplasma gondii seropositivity and risk factors in pregnant women followed up by the Family Health Strategy.

Introduction: Toxoplasmosis is a zoonotic infection caused by Toxoplasma gondii. It is transmitted by the ingestion of contaminated water and foods, by soil contaminated with cat feces, especially while handling it, and congenitally via the placenta. The diagnosis of maternal infection is made by serological detection of either IgM or IgG antibodies.

Capillaria hepatica in Puma concolor: first report in Brazil.

Capillaria hepatica was detected by histopathologic diagnosis in two cougars that were shot in April 2008 in Painel, Santa Catarina, Brazil. Macroscopic analysis of their livers revealed the presence of diffuse granulomas, and the histopathologic analysis indicated the presence of C. hepatica eggs, surrounded by mononuclear inflammatory cell infiltrate, small foci of necrosis, and mild-to-moderate fibrosis.

Presence of Fasciola hepatica in feral nutria (Myocastor coypus) living in a public park in Brazil.

Sixteen nutria (Myocastor coypus) from a protected area in Curitiba, Brazil, were sampled to determine the prevalence of Fasciola hepatica eggs and intestinal parasites and the presence of snails in the habitat used by nutria. The overall prevalence rates were 56.25% (9) for F.

Glomerulonephritis in water buffaloes (Bubalus bubalis) naturally infected by Fasciola hepatica.

Glomerulonephritis caused by Fasciola hepatica was observed in buffaloes. Renal biopsies of 20 buffaloes, 11 with F. hepatica and 9 uninfected buffaloes (controls), were examined by light microscopy, direct and indirect immunofluorescence, and immunohistochemical analysis.

Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin.

A growing interest in the understanding of the ontogeny and mineralization of fish skeleton has emerged from the recent implementation of fish as a vertebrate model, particularly for skeletal development. Whereas several in vivo studies dealing with the regulation of bone formation in fish have been published, in vitro studies have been hampered because of a complete lack of fish-bone-derived cell systems. We describe here the development and the characterization of two new cell lines, designated VSa13 and VSa16, derived from the vertebra of the gilthead sea bream.

Restriction of human immunodeficiency virus type 1 Rev function in murine A9 cells involves the Rev C-terminal domain.

The human immunodeficiency virus type 1 (HIV-1) Rev and human T-cell leukemia virus type 1 (HTLV-1) Rex proteins are essential for the expression of viral structural proteins and productive infection. Both contain a nuclear export signal (NES) in their C-terminal domain and a nuclear localization signal (NLS) in their N-terminal domain. The NES and NLS are necessary for shuttling between nucleus and cytoplasm and are therefore indispensable for the transport of unspliced and singly spliced viral transcripts.