Using the Teaching Pyramid Observation Tool to Support Professional Development Experiences in Public 4 K Settings.

This study examines how well school groups in the Coastal Plains region of a southeastern state align with the socio-emotional strategies for four-year-olds outlined by the Key Practices in the Pyramid Model Framework (Teaching Pyramid Observation Tool-Research Edition [Manual], Baltimore, MD: Brookes, 2014). Many schools in these groups serve children in poverty, so it is imperative that teachers engage in developmentally appropriate socioemotional practices. The authors used the TPOT observation tool to take baseline data and examine longitudinally after giving ongoing professional development experience sessions.

Identifying the Needs of Prekindergarten Children: A Focus on Health, Wellbeing, and Family Environment.

The impact of children's health, wellbeing, and family environment on their learning and development is evident. Identifying the needs of children and their families is the first step to effectively developing and implementing programs that promote child development. This research employed a convergent mixed methods design and incorporated multiple data collection and analysis techniques to explore the needs of prekindergarten children related to their health, wellbeing, and family environment.

Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing.

Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes that deaminate adenosine to inosine. Although many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass spectrometry to identify trans regulators of ADAR1 and ADAR2 in HeLa and M17 neuroblastoma cells.

Protein recoding by ADAR1-mediated RNA editing is not essential for normal development and homeostasis.

Background: Adenosine-to-inosine (A-to-I) editing of dsRNA by ADAR proteins is a pervasive epitranscriptome feature. Tens of thousands of A-to-I editing events are defined in the mouse, yet the functional impact of most is unknown. Editing causing protein recoding is the essential function of ADAR2, but an essential role for recoding by ADAR1 has not been demonstrated.

Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells.

One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is the inability to identify the developmental stage of these cells without the need for genetic manipulation or utilization of exogenous markers. In this study, we demonstrate that Raman microspectroscopy can non-invasively identify embryonic stem cell (ESC)-derived chamber-specific CMs and monitor cell maturation. Using this marker-free approach, Raman peaks were identified for atrial and ventricular CMs, ESCs were successfully discriminated from their cardiac derivatives, a distinct phenotypic spectrum for ESC-derived CMs was confirmed, and unique spectral differences between fetal versus adult CMs were detected.

Understanding contextual influences of community reintegration among injured servicemembers.

As part of a larger mixed-methods research project investigating the influence of contextual factors on community reintegration (CR), this qualitative study sought to understand the subjective experiences of injured servicemembers and their perception of how contextual factors influenced their CR. More specifically, this article addresses how the influences of contextual factors differ between injured servicemembers with different levels of CR. Using a phenomenological framework, semistructured interviews were conducted with nine injured, community-dwelling servicemembers with low, moderate, and high levels of CR (three per category).

Identifying contextual influences of community reintegration among injured servicemembers.

Research suggests that community reintegration (CR) after injury and rehabilitation is difficult for many injured servicemembers. However, little is known about the influence of the contextual factors, both personal and environmental, that influence CR. Framed within the International Classification of Functioning, Disability and Health and Social Cognitive Theory, the quantitative portion of a larger mixed-methods study of 51 injured, community-dwelling servicemembers compared the relative contribution of contextual factors between groups of servicemembers with different levels of CR.

The Effects of Yoga Participation on Women's Quality of Life and Symptom Management During the Menopausal Transition: A Pilot Study.

Typically lasting 5-10 years, the menopausal transition is associated with symptoms including hot flashes, night sweats, and labile mood. As these symptoms often hinder a woman's successful functioning in everyday life, hormone therapy is commonly prescribed as a means to diminish symptoms. Many women, however, are seeking complementary and alternative treatments due to side effects and/or detrimental health-risks associated with conventional therapies.