Immunocytochemistry response of a hybrid (chitosan--glycidyl methacrylate)-xanthan scaffold for cell proliferation.

In the present work, chitosan-glycidyl methacrylate-xanthan ((CTS--GMA)-X) hydrogel was successfully synthesized by the chemical reaction of a co-polymer of chitosan--glycidyl methacrylate (CTS--GMA), under different stoichiometric molar ratios from 1 : 1 to 1 : 4, and xanthan (X). (CTS--GMA)-X was synthesized by two methods to obtain a hydrogel: an aqueous acid media followed by neutralization and a neutral aqueous media, with an extended reaction time. Human epidermal keratinocytes (HEK) and nerve cells (neuroblastoma × glioma hybrid cell NG108-15) were sown over the hydrogels, and their vitality was determined using a calcein stain.