Impact of Modified Atmospheres on Growth and Metabolism of Meat-Spoilage Relevant spp. as Predicted by Comparative Proteomics.

Modified atmosphere packaging (MAP) is a common strategy to selectively prevent the growth of certain species of meat spoiling bacteria. This study aimed to determine the impact of high oxygen MAP (70% O, 30% CO, red and white meats) and oxygen-free MAP (70% N, 30% CO, also white meat and seafood) on preventing the growth of spoiling photobacteria on meat. Growth of and was monitored in a meat simulation media under different gas mixtures of nitrogen, oxygen, and carbon dioxide, and samples were taken during exponential growth for a comparative proteomic analysis.

Comparative genomics of species from terrestrial and marine habitats.

() is a genus widely studied in regards to its association with and ubiquitous presence in marine environments. However, certain species () have been recently described to colonize and spoil raw meats without a marine link. We have studied 27 strains from meat as well as 26 strains from marine environments in order to probe for intraspecies marine/terrestrial subpopulations and identify distinct genomic features acquired by environmental adaptation.

Development of a rapid detection method for Photobacterium spp. using Loop-mediated isothermal amplification (LAMP).

While the abundance of photobacteria has previously been exclusively associated with marine environments and spoilage of seafood, several recent studies have demonstrated their status as pervasive constituents of the microbiota on packaged meats. Since their ubiquitous nature has been revealed, detection of their presence on meat, their entry route into meat processing environments and prevention of their growth is a novel emerging challenge for the food industry. In this study, we have developed a highly sensitive and specific loop-mediated isothermal amplification (LAMP) assay for the detection of relevant species of photobacteria on foods, and tested its efficacy on meats.

Biodiversity of spp. Isolated From Meats.

Photobacteria are common psychrophilic bacteria found in marine environments. Recently, several studies revealed high numbers of () spp. on packaged fresh meat.