Publications by authors named "Sandra A Olivier"
Front Microbiol
December 2023
Article Synopsis
- - Lactic acid bacteria play crucial roles in various industries, and accurately identifying their species from mixed environments is essential but challenging due to genetic similarities among species.
- - Traditional amplicon-based methods, like short-read sequencing of the 16S rRNA gene, often struggle with species-level resolution, which can be improved by using long-read sequencing (LRS) techniques.
- - In experiments comparing different amplicons, LRS using V3-V4 yielded lower accuracy in species identification and higher false positives compared to complete 16S rRNA gene and 16S-ITS-23S rRNA region amplicons, emphasizing the need for careful method selection in microbial community analysis.
