Treatment of clinical laboratory sewage using a decentralized treatment unit and risk reduction for its reuse in irrigation using hybrid disinfection.

The disinfection efficacy of standalone chlorine, UV and their combined approach (hybrid) was investigated for the coliform removal in BioKube 1 and 2 treated effluents collected from different environmental settings of clinical and domestic wastes. Chlorine and UV disinfection were applied to BioKube treated wastewater with doses from 0 to 4 mg L and 0-166 mJ cm respectively. Combined disinfection strategies were designed to reduce the dose of chlorine and UV and to exploit the synergistic effect of them.

Antibiotic resistance genes and bacterial diversity: A comparative molecular study of treated sewage from different origins and their impact on irrigated soils.

Present study aims to investigate how is soil affected following irrigation with treated effluents of different origins by analysing the bacterial diversity, metabolic diversity and antibiotic resistance genes (ARGs). Comparative analysis with previously reported ARGs in effluents was performed to understand the mobility of ARGs from treated wastewater to the irrigated soil with respect to the control soil regimen. Acinetobacter, Burkholderia and Pseudomonas were observed as the most abundant genera in all the samples.

A systematic review on chlorine tolerance among bacteria and standardization of their assessment protocol in wastewater.

Though chlorine is a cost-effective disinfectant for water and wastewaters, the bacteria surviving after chlorination pose serious public health and environmental problems. This review critically assesses the mechanism of chlorine disinfection as described by various researchers; factors affecting chlorination efficacy; and the re-growth potential of microbial contaminations in treated wastewater post chlorination to arrive at meaningful doses for ensuring health safety. Literature analysis shows procedural inconsistencies in the assessment of chlorine tolerant bacteria, making it extremely difficult to compare the tolerance characteristics of different reported tolerant bacteria.

Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater.

Alongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains poorly described. In this study, we used metagenomic sequencing to characterize ARGs and biocide/metal resistance genes (BMRGs) in four wastewater treatment plants (WWTPs) in Jaipur City of India.

Impact of combined chlorine and UV technology on the bacterial diversity, antibiotic resistance genes and disinfection by-products in treated sewage.

This paper explores the effect of hybrid chlorine and UV disinfection treatment against their individual usage on microbial community, functional genes, antibiotic resistant genes (ARGs) and disinfection by-products (DBPs) formation. The disinfectant doses of 2.5 mg L chlorine and 41 mJ cm UV were selected based on the coliform counts to be attained in treated sewage.

Investigation of chlorine tolerance profile of dominant gram negative bacteria recovered from secondary treated wastewater in Jaipur, India.

Management of bacterial survival post chlorine disinfection is vital for safe wastewater reuse for irrigation, as the presence of microorganisms in large numbers may lead to subsequent contamination of the surface and ground water reservoirs. Even after satisfying the current norms of coliform counts after disinfection (less than 1000 MPN per 100 mL), chlorine tolerant bacteria surviving in inadequately treated wastewater may pose a public health threat as many of these bacteria are able to re-grow upon storage. The current study is aimed to assess the magnitude of the problem posed by chlorine tolerant bacteria during chlorine disinfection and attempts to derive a strategy for safe disinfection.