Human sodium current voltage-dependence at physiological temperature measured by coupling a patch-clamp experiment to a mathematical model.

Voltage-gated Na channels are crucial to action potential propagation in excitable tissues. Because of the high amplitude and rapid activation of the Na current, voltage-clamp measurements are very challenging and are usually performed at room temperature. In this study, we measured Na current voltage-dependence in stem cell-derived cardiomyocytes at physiological temperature.