Publications by authors named "Samuel Pritt"
Article Synopsis
- Developed a new method to measure protein binding affinity using microscale thermophoresis (MST) without needing purified proteins, making it quicker and less costly.
- The protocol involves overexpressing a GFP-fused protein and lysing cells under gentle conditions, allowing for easier testing of interactions.
- Successfully applied this method to determine the binding affinity of the transcription factor STAT3-GFP to various DNA sequences, which could open new avenues for studying diverse protein interactions.
