aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration.

Apical-basal polarity is a hallmark of epithelia and needs to be remodeled when epithelial cells undergo morphogenetic cell movements. Here, we analyze border cells in the ovary to address how apical-basal polarity is remodeled and turned into front-back and inside-outside as well as apical-basal polarities, during collective migration. We find that the Crumbs (Crb) complex is required for the generation of the three distinct but interconnected cell polarities of border cells.