Using the dCas9-KRAB system to repress gene expression in hiPSC-derived neurons.

We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ) in human induced pluripotent stem cell-derived -induced glutamatergic neurons.

National, Virtual Otolaryngology Training Day in the United Kingdom During the COVID-19 Pandemic: Results of a Pilot Survey.

The COVID-19 pandemic has forced an unprecedented worldwide change in our daily lives, particularly in healthcare. In response to the lockdown measure it has brought on, some deaneries cancelled planned teaching days, while others had delivered remote smaller scale teaching sessions electronically. Due to significant regional variation, the National Association of Program Directors in ENT (NAPDENT) set up a national educational program, matched to the Intercollegiate Surgical Curriculum Program.

Ancient schwannoma of the cervical sympathetic chain.

Ancient schwannomas exhibit characteristic histological features. Fine-needle aspiration cytology (FNAC) is of limited use. Radiological evidence demonstrating displacement of blood vessels may aid diagnosis of schwannoma.

Presentation of Mycobacterium abscessus infection following rhytidectomy to a UK plastic surgery unit.

We report the presentation of a patient to a UK plastic surgery unit with Mycobacterium abscessus infection following a facelift surgery in Southern India. Treatment was protracted requiring surgical debridement and 6 months of antibiotics including a 3-week hospital admission for intravenous antibiotic therapy. We describe the clinical presentation, diagnosis and treatment of this unusual microorganism with reference to more familiar pyogenic infections.