Factors Affecting Spontaneous Endocytosis and Survival of Probiotic Lactobacilli in Human Intestinal Epithelial Cells.

Mutualistic bacteria have different forms of interaction with the host. In contrast to the invasion of pathogenic bacteria, naturally occurring internalization of commensal bacteria has not been studied in depth. Three in vitro methods, gentamicin protection, flow cytometry and confocal laser scanning microscopy, have been implemented to accurately assess the internalization of two lactobacillus strains- BL23 and GG-in Caco-2 and T84 intestinal epithelial cells (IECs) under a variety of physiological conditions and with specific inhibitors.

A Change in Conservation Status of (Crassulaceae), a Threatened Species from Central Mexico Based on Genetic Studies.

Genetic studies in rare species are important to determine their patterns of genetic diversity among populations and implement conservation plans aimed to reduce extinction risks. is an endemic species with extremely small populations in central Mexico. This work analyzes the diversity and genetic structure of (Crassulaceae) to change the conservation status and propose conservation strategies.

Drug resistance and phylogenetic grouping of bacteria isolated from visitors' hands in a secondary-care hospital.

Introduction: Dynamic movement in the hospital environment promotes the transmission of nosocomial pathogens and multidrug resistance mechanisms through the dissemination of organisms that carry genetic determinants. Healthcare workers play an important role in the spread of pathogens; however, the role of visitors in this environment is poorly understood.

Objective: This study aimed to molecularly identify and examine the antibiotic resistance of the palmar microbiota of patients' companions in a hospital waiting room.

Evaluation of nest management phases for Lepidochelys olivacea at two beaches in Northwest Mexico.

The olive ridley turtle, Lepidochelys olivacea, is a vulnerable and endangered species according to the IUCN and Mexican Official Standard NOM-059, respectively. On most solitary nesting beaches of olive ridley turtles, the eggs are removed from the in situ nest to hatcheries due to the high incidence of predation, human poaching, and beach erosion; therefore, it is necessary to collect and analyze information on the protection activities conducted for this species from egg laying to hatchling release. In general, protection activities during nest management can be divided into 5 phases: nest logging (F1), egg collection (F2), egg transfer (F3), egg incubation and hatching (F4), and hatchling release (F5).

Determination of drug-resistant bacteria in palmar surface and touchscreen cell phones from bystanders in an urban community.

The dynamic microbiota of the human palmar surface (PS) is related to the various hygienic habits of humans and can be transmitted or exchanged upon contact with objects of daily use, such as the indispensable touchscreen cell phone (TCP); this interaction could allow the development of drug-resistant bacteria. The objective was to determine the drug-resistant bacterial contamination between PSs and TCPs in an urban community. Among the total bacterial colonies isolated and molecular and phylogenetically characterized based on the V4-V6 regions of the 16S rRNA gene from PSs and TCPs, the genera present in both types of samples were Staphylococcus (53.

Phylogenetic characterization and multidrug resistance of bacteria isolated from seafood cocktails.

The continual increase in resistance to antibacterial drugs has become a major public health problem, and their indiscriminate use in agriculture, aquaculture, and the treatment of human and animal diseases has severely contributed to the occurrence and spread of multidrug resistance genes. This study phylogenetically characterized multidrug-resistant bacteria isolated from seafood cocktails. Seafood cocktail dishes from 20 establishments on public roads were sampled.

Isolation of Gram-Positive, Antibiotic-Resistant Bacteria from Tactile Mobile Phones in a Northwestern Mexican City.

The widespread use of touch-screen mobile devices renders them potential fomites for the transmission of bacterial pathogens among users of different ages. The objectives of the present research were to isolate bacteria from mobile phones, perform molecular and phylogenetic identification, and determine the antibiotic resistance profiles. The surfaces of 50 touch-screen mobile devices owned by bystanders were sampled in the city center of Culiacan, Sinaloa, Mexico.

Identification of immunodominant peptides from Gnathostoma binucleatum.

Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of Gnathostoma binucleatum and in Asia mainly by G. spinigerum.

Is Gnathostoma turgidum an annual parasite of opossums? Drastic seasonal changes of infection in Didelphis virginiana in Mexico.

Gnathostoma turgidum is a nematode that parasitizes the stomach of opossums, Didelphis virginiana. Despite its wide distribution in the Americas, its natural life cycle is poorly understood. Recently, we found an endemic area for G.

Identification of estuarine fish Dormitator latifrons as an intermediate host and Eleotris picta as a paratenic host for Gnathostoma binucleatum in Sinaloa, Mexico.

Gnathostomosis is a typical fish-borne zoonotic parasitosis and is currently a serious public health issue in Mexico. Among several Gnathostoma species present in wild animals in Mexico, Gnathostoma binucleatum is the only proven species responsible for human diseases, and the advanced third stage larvae (AL3) of G. binucleatum have been found in over 20 species of fish in this country.

Physicochemical and nutritional characteristics of the fruit of Zizyphus sonorensis S. Wats (Rhamnaceae).

The nanche de la costa (Zizyphus sonorensis) fruit was partially characterized by physicochemical and nutritional analyses. This fruit has a homogenous size and color and low moisture content (47 g/100 g). The content of tannins and nickel of the edible portion might limit its use.