ER exit sites (ERES) and ER-mitochondria encounter structures (ERMES) often localize proximally.

To understand the potential interplay between vesicular trafficking and direct membrane contact sites-mediated transport, we selected the endoplasmic reticulum (ER), which participates in both modes of inter-organelle transport. ER-mitochondria encounter structures (ERMES) are direct membrane contact junctions that mediate macromolecule exchange, while the secretory pathway originates at ER exit sites (ERES). Using the budding yeast Pichia pastoris, we documented that ERMES resident proteins are often juxtaposed with ERES markers.