[Effects of ubiquinone-9 on lipids of nuclei and chromatin of the rat liver under continuous irradiation].

The influence of continuous gamma irradiation on the lipids of nuclei and chromatin of rat liver at a dose-rate of 0,129 Gy/day for 155 days (a total dose of 20 Gy) and by feeding of ubiquinone-9 has been studied. The amount of phosphatidylcholine with phosphatidylserine and phosphatidyl-ethanolamine in liver nuclei of irradiated rats was found to increase. Ubiquinone-9 had a normalizing effect.

[Cardioprotective effect of combined use of coenzyme Q9 and cyclohexyladenosine in ischemia, reperfusion and acute myocardial infarction].

Effects of coenzyme Q9 (25 mg/kg), N6-cyclohexyl adenosine (CHA, 100 micrograms/kg) and their combination were compared in rats with short-term or permanent ligation of the left coronary artery. The following parameters were evaluated in three series of experiments: 1) incidence and duration of ventricular fibrillation and tachycardia during coronary occlusion (10 min) and consecutive reperfusion (5 min); 2) contractility and electrical stability of the heart (ventricular fibrillation threshold) in animals with 2-day myocardial infarction; 3) ischemic myocardial mass after coronary occlusion (5 min) and necrotic tissue mass in 2-day myocardial infarction. The rats were given oral drugs 5 days and 2 hours before the study.

[Radioprotective properties of ubiquinones during acute and chronic irradiation of rats].

Protective and therapeutic effect of ubiquinone-8 was demonstrated on rats exposed to 8 Gy radiation. There was a 16-20% increase in the survival rate of the irradiated rats as compared with the controls. The per os administration of ubiquinone-8 during a five-month period in conditions of chronic exposure arrested the radiation-induced accumulation of lipids in rat liver and somewhat improved the dynamics of the body mass growth in the exposed rats.

[Effect of three retinoids on the growth of experimental tumors].

Influence of retinoid C17-diester retinyldimedone and L-tocopherylretinoate on the growth of transplanted tumors was investigated in F1 (C57Bl/6 X CBA) strain of mice. The retinoids studied proved to be less toxic than retinoic acid. Both retinoid C17-diester and L-tocopherylretinoate reduced growth of cervical cancer, while retinyldimedone inhibited growth of Lewis carcinoma (lung tumor) and of stomach carcinoma.

[Effect of ubiquinone-9 on the blood coagulation system].

The effect of a single and 2-week administration of ubiquinone-9 on the blood coagulation system and the functional activity of platelets was studied in the experiments in vitro and in vivo on rats. It was shown that a single dose of 150 mg/kg of ubiquinone decreased adhesion and that of 5 mg/kg decreased aggregation of platelets. Both with a single and 2-week drug administration in doses of 5, 50 and 150 mg/kg an insignificant decrease of coagulability was observed.

[Effect of ubiquinone on phospholipid metabolism in radiation injury].

The synthesis and phospholipid content in the liver, intestine and spleen in normal and irradiated rats administered ubiquinone-9 were studied with the use of 3H-serine. Ubiquinone markedly activated decarboxylation of phosphatidylserine and suppressed transformation of phosphatidylethanolamine to phosphatidylcholine in rat liver and spleen. The effect was also observed in the organs of irradiated animals.

[Therapeutic effect of vegetable oils and ubiquinone-9 on radiation injuries].

The comparison was made of the protective (the administration 3 h before irradiation with a dose of 7.3 Gy) and therapeutic (the administration immediately and later after exposure) effects of soya oil (150 mg/kg) and oil solution of ubiquinone-9 (100-200 mg/kg) on survival of exposed rats. It was shown that soya oil and ubiquinone-9 increased the survival rate of rats when administered before and, to a lesser extent, immediately after irradiation.

[Ubiquinone-9 and ergosterol from Candida paralipolytica yeasts].

Ubiquinone-9 and ergosterol were extracted with hexane from the yeast Candida paralipolytica after hydrolysis with KOH in EtOH with the yield 0.026 and 0.14%, respectively.

[Effect of synthetic beta-carotene on the formation of cytolytic T-lymphocytes].

The effect of intraperitoneal injection of beta-carotene in different doses on the formation of cytolytic T lymphocytes (CTL) in a one-way mixed lymphocyte culture (MLC) of allogeneic mice was studied. The maximal cytotoxic activity of lymphocytes was attained in the MLC with splenocytes of mice which received 10 mg/kg beta-carotene 6 days before experimentation. The correlation was studied between the beta-carotene ability to stimulate CTL formation and antineoplastic activity.

[Protective and therapeutic properties of the ubiquinones H6CoQ4 and CoQ9 in gamma irradiation].

Hexahydroubiquinone-4 (H6CoQ4) and ubiquinone-9 (CoQ9) in the form of solutions in vegetable oil administered to mice and rats subcutaneously and intragastrically produced a radioprotective effect comparable with that of cystamine. Under the accepted experimental conditions the compounds did not produce any therapeutic effect.

[Ubiquinone-9 of the flagellates Crithidia oncopelti and Astasia longa].

The content of ubiquinones (Co Q) of the Astasia longa and Crithidia oncopelti protozoa was studied. The protozoa were grown on an artificial nutrient broth. The cells were separated, washed, freeze-dried, and refluxed with KOH and pyrogallol in ethanol media.

[Effect of retinoids on the development and growth of experimental tumors].

The ability of six retinoids to inhibit cancer growth was tested on mice with Lewis lung carcinoma, PRG and RSM-5 tumors. It was shown that all tumors possess individual sensitivities to retinoids. The most effective substances were: methyl retinoate (all-trans and 13-cis isomers) and a retinoid with a small side chain and an oxidated beta-iononic ring (substance 3).

[Effect of native vitamin A metabolites and their 4-oxo derivatives on formation of specific cytolytic T lymphocytes].

Immunomodulating effect of some structural derivatives of retinoic acid (RA) on formation of specific cytolytic T lymphocytes (T killers) was studied in mixed cell culture of spleen lymphocytes from allogenic mice. Native vitamin A metabolites (RA, 13-cis-RA) and the structure-like substances (C15-C20-retinoids and others) at 10(-6) = 10(-7) M concentrations increased 2-2.5-fold the formation of T killers in vitro after stimulation of the lymphocytes by suboptimal doses of allogenic X-ray irradiated cells, the lymphocyte proliferation was inhibited in the mixed culture at 10(-5) M and higher concentrations of the substances.

[Toxicological and pharmacological properties of ubiquinone-9 and hexahydroubiquinone-4].

Experiments were made on random-bred and linear mice, Wistar rats, rabbits and dogs to examine pharmacological properties of ubiquinone-9 and hexahydroubiquinone-4. Both compounds (oily solutions) administered intraperitoneally, intragastrically and subcutaneously in single and multiple doses produced no changes in the behavior or status of the animals, which might have attested to their toxicity. They were found to exert a regulatory action on the central nervous system, decreasing its sensitivity to numerous narcotic and convulsant substances without appreciable effects on the blood, arterial pressure, ECG and diuresis.

[Antitumor and toxic properties of retinoid C15].

A study was made of antitumor and toxic properties of retinoid C15 which has some characteristic structural features of retinoic acid natural metabolites; such as ethyl-2E, 4E-3-methyl-5-/2,6-dimethyl-6-ethoxycarbonyl-/3-oxo-1-cyclohexene-1-yl/-2,4-pentadienoate. The substance inhibited the growth of transplantable tumors of the uterine cervix. Lewis carcinoma and rumen cancer.

[Comparative study of the biological activity and toxic effect of 1alpha-hydroxycholecalciferol and ergocalciferol in rats].

Single administration of 0.25 microgram of sunthetic Ialpha-hydroxycholecalciferol (IalphaOHD3) into nephrectomized rats, maintained at D-avitaminous diet, improved the active transport of calcium ions against the concentration gradient in small intestine of these animals, whereas ergocalciferol was biologically inactive under the same conditions. Administration of IalphaOHD3 during 5 days at a dose 0.

[Effect of hexahydroubiquinone-4 (KoQ4) on the bioenergetics and functional activity of the myocardium in ischemia].

The effect of KoQ4 on the energetics, contractility, and electrogram of the ischemized myocardium was studied in acute experiments on dogs with induced myocardial ischemia. Intracornoary administration of KoQ4, 1.3 mg/kg, directly into the focus of ischemia for 15 min promoted a decrease in the lactate level in blood draining from the ischemic zone as compared to the control data in the absence of a difference in the dynamics of the pyruvic acid content.

[Structural changes in ganglioside micelles during interaction with serotonin].

Structure changes of ganglioside micelles in the process of serotonin bonding are considered. It was shown by viscosimetric and differential refraction studies that stretching of elliptical ganglioside micelles in the serotonin bonding process took place. The scope of micelles form changes depends on the pH value.