PHYSICAL HEALTH OF FEMALES FROM THE LOWLAND DISTRICTS OF ZAKARPATTIA ACCORDING TO THE METABOLIC LEVEL OF AEROBIC AND ANAEROBIC ENERGY SUPPLY DEPENDING ON THE COMPONENT BODY COMPOSITION.

Objective: The aim: To determine the aerobic and anaerobic productivity of females from the lowland districts of Zakarpattia region, depending on the component composition of body weight.

Patients And Methods: Materials and methods: A comparative analysis of physical health status of females in the post-pubertal period of ontogenesis, was carried out. Physical health status was assessed by indicators of aerobic and anaerobic productivity depending on the component composition of the body, which was determined by impedance measurement.

Design of a modular DSS for public health decision-making in the context of a COVID-19 pandemic landscape.

The awareness of the occurrence of a new disease involves much uncertainty and the search for answers and also appropriate questions. In this paper we focus on the perspective of public health decision-makers. Typically, they would have a standard set of questions and supporting metrics that have been found in previous disease outbreaks to be useful in assessing the effectiveness of various 'solution' methods on the trajectory of the disease.

[RNA, unlike DNA, binds to aurintricarboxylic acid via covalent bonds: molecular-biological and spectroscopic evidence].

A selective interaction of ATA with RNA, unlike DNA, when isolating nucleic acids from plant materials was established. The data concerning the binding strength of highly purified RNAATA and DNAATA preparations to nitrocellulose and nylon filters under conditions of high ionic strength are presented. The interrelation of ATA RNA-tropism and absence of adsorption capability of chemical modified RNAATA the backbone was observed.

Characteristics of the hypoosmosis-induced calcium response in isolated nerve terminals of rat brain.

Background: Cerebral edema contributes significantly to morbidity and death in many common neurological disorders. Ca2+ ions play a important role in the development of pathophysiological reactions in nerve cells. The aim of our research was to elucidate the mechanism of the osmotically-induced entrance of Ca2+ into nerve terminals (synaptosomes) and to estimate the involvement of endoplasmic reticulum Ca2+ stores in intrasynaptosomal Ca2+-dependent processes in hypoosmotic swelling.

[Use of excimerization of pyrene for assessing lipid microviscosity in biological membranes].

A method for estimating the fluidity of natural membranes from the pyrene excimer/monomer fluorescence ratio (Ie/Im) is proposed. The method makes it possible to exclude artefacts such as fluorescence quenching, aggregation, and redistribution of the probe in lipid mains with different microviscosity. It is shown that, upon variation of intramembrane pyrene concentration [pyr], the occurrence of a common crossover point in pyrene fluorescence spectra normalized to the corresponding probe concentration (isoemission or isobestic point) or, as a consequence, the linear dependence of Ie/[pyr] on Im/[pyr] can serve as a criterion of diffusion (fluidity)-controlled excimerization of pyrene.

[Effect of ischemic damage factors on lipid peroxidation in rat brain synaptosomes].

Accumulation of TBA-reactive substances after a 40 min incubation of rat brain synaptosomes at 37 degrees C was analysed. A lowering of pH to 6.5-5.

Effect of proteolysis on the state of lipid phase in rat brain synaptosomal membranes.

Enzymatic proteolysis of the proteins of synaptic membranes has been found to be accompanied by the promotion of lipid peroxidation probably mediated by the liberation of membrane-bound iron. As fluorescent probes pyrene and diphenylhexatriene show, the microviscosity and micropolarity of membrane lipid phase rise as a result of lipid peroxidation. Different structural changes induced by proteolysis are displayed under inhibition of lipid peroxidation.

Effect of gamma-irradiated carbohydrates on isolated synaptic membranes.

The effect of gamma-irradiated solutions of carbohydrates, mainly glucose, upon Na+, K(+)-ATPase and lipid peroxidation in rat brain synaptosomal membranes was studied. The membrane damage by irradiated glucose was enhanced in the presence of Fe2+ and was diminished when a free-radical scavenger (BHT) or metal chelators (EDTA, EGTA) were present. It is suggested that a key element in the free-radical membrane damage by irradiated carbohydrates is an Fe(2+)-complex of some species of the radiolysis products.

[Effect of temperature on polarity of an annular and bilayer synaptic membrane lipid].

Using fluorescent probe pyrene a study was carried out of temperature dependence of the annular and lipid bilayer polarity in synaptic membranes. Polarity of microenvironment was evaluated by a ratio of intensities of vibronic bands in pyrene fluorescence spectra at direct excitation of probe or through energy transfer from protein molecules. At the temperature range 10-50 degrees C the polarity of bilayer was shown to increase while that of annular lipid underwent biphasic changes.

[Spectroscopic study of interactions between nucleic acid bases and amino acid esters in dimethylsulfoxide].

Interactions between nucleic acid bases (A, T, U. C) and some amino acid esters have been investigated by IR and UV spectroscopies in dimethylsulfoxide. The stability of associates of amino acid esters with the bases was established to form the following sequence: C greater than A much greater than U, T.