The murine metastatic microenvironment of experimental brain metastases of breast cancer differs by host age in vivo: a proteomic study.

Breast cancer in young patients is known to exhibit more aggressive biological behavior and is associated with a less favorable prognosis than the same disease in older patients, owing in part to an increased incidence of brain metastases. The mechanistic explanations behind these findings remain poorly understood. We recently reported that young mice, in comparison to older mice, developed significantly greater brain metastases in four mouse models of triple-negative and luminal B breast cancer.

WGS-based screening of the co-chaperone protein DjlA-induced curved DNA binding protein A (CbpA) from a new multidrug-resistant zoonotic mastitis-causing Klebsiella pneumoniae strain: a novel molecular target of selective flavonoids.

The research aimed to establish a multidrug-resistant Klebsiella pneumoniae-induced genetic model for mastitis considering the alternative mechanisms of the DjlA-mediated CbpA protein regulation. The Whole Genome Sequencing of the newly isolated K. pneumoniae strain was conducted to annotate the frequently occurring antibiotic resistance and virulence factors following PCR and MALDI-TOF mass-spectrophotometry.

Easy synthesis of PPy/TiO/ZnO composites with superior photocatalytic performance, efficient supercapacitors and nitrite sensor.

The synthesis of Polypyrrole (PPy)/TiO/ZnO composites involved a chemical oxidative polymerization process, wherein the addition of TiO/ZnO was varied from 1 to 10 wt%. The composites' photocatalytic capabilities, supercapacitor performance, and potential use as a nitrite sensor were thoroughly assessed, alongside investigations into their photoluminescence (PL) and morphological characteristics. The strong interaction between TiO/ZnO and PPy was confirmed using FTIR, UV-Vis, and PL spectroscopy techniques.

Target-specificity of different amyrin subunits in impeding HCV influx mechanism inside the human cells considering the quantum tunnel profiles and molecular strings of the CD81 receptor: a combined in silico and in vivo study.

HCV is a hepatotropic RNA virus recognized for its frequent virulence and fatality worldwide. Despite many vaccine development programs underway, researchers are on a quest for natural bioactive compounds due to their multivalent efficiencies against viral infections, considering which the current research aimed to figure out the target-specificity and therapeutic potentiality of α, β, and δ subunits of amyrin, as novel bioactive components against the HCV influx mechanism. Initially, the novelty of amyrin subunits was conducted from 203 pharmacophores, comparing their in-silico pharmacokinetic and pharmacodynamic profiles.

Biochemical and structural characterization of analogs of MRE11 breast cancer-associated mutant F237C.

The MRE11-RAD50-NBS1 (MRN) protein complex plays a vital role in DNA double strand break sensing, signaling, and repair. Mutation in any component of this complex may lead to disease as disrupting DNA double strand break repair has the potential to cause translocations and loss of genomic information. Here, we have investigated an MRE11 mutation, F237C, identified in a breast cancer tumor.

Automatic identification of abnormal blood smear images using color and morphology variation of RBCS and central pallor.

The anatomy of red blood cells (RBCs) in blood smear images plays an important role in the detection of several diseases. The automated image-based technique is fast and accurate for the analysis of blood cells morphology that can save time of both pathologists as well as that of patients. In this paper, we propose a novel method which segment and identify varied RBCs in a given blood smear images.

A Survey of Reported Disease-Related Mutations in the MRE11-RAD50-NBS1 Complex.

The MRE11-RAD50-NBS1 (MRN) protein complex is one of the primary vehicles for repairing DNA double strand breaks and maintaining the genomic stability within the cell. The role of the MRN complex to recognize and process DNA double-strand breaks as well as signal other damage response factors is critical for maintaining proper cellular function. Mutations in any one of the components of the MRN complex that effect function or expression of the repair machinery could be detrimental to the cell and may initiate and/or propagate disease.

Mutation of Conserved Mre11 Residues Alter Protein Dynamics to Separate Nuclease Functions.

Naked and protein-blocked DNA ends occur naturally during immune cell development, meiosis, and at telomeres as well as from aborted topoisomerase reactions, collapsed replication forks, and other stressors. Damaged DNA ends are dangerous in cells and if left unrepaired can lead to genomic rearrangement, loss of genetic information, and eventually cancer. Mre11 is part of the Mre11-Rad50-Nbs1 complex that recognizes DNA double-strand breaks and has exonuclease and endonuclease activities that help to initiate the repair processes to resolve these broken DNA ends.

Radar micro-Doppler signatures of drones and birds at K-band and W-band.

Due to the substantial increase in the number of affordable drones in the consumer market and their regrettable misuse, there is a need for efficient technology to detect drones in airspace. This paper presents the characteristic radar micro-Doppler properties of drones and birds. Drones and birds both induce micro-Doppler signatures due to their propeller blade rotation and wingbeats, respectively.

A dynamic allosteric pathway underlies Rad50 ABC ATPase function in DNA repair.

The Mre11-Rad50 protein complex is an initial responder to sites of DNA double strand breaks. Many studies have shown that ATP binding to Rad50 causes global changes to the Mre11-Rad50 structure, which are important for DNA repair functions. Here we used methyl-based NMR spectroscopy on a series of mutants to describe a dynamic allosteric pathway within Rad50.