AUF-1 knockdown in mice undermines gut microbial butyrate-driven hypocholesterolemia through AUF-1-Dicer-1-mir-122 hierarchy.

Introduction And Objective: Cholesterol homeostasis is a culmination of cellular synthesis, efflux, and catabolism to important physiological entities where short chain fatty acid, butyrate embodied as a key player. This discourse probes the mechanistic molecular details of butyrate action in maintaining host-cholesterol balance.

Methods: Hepatic mir-122 being the most indispensable regulator of cholesterol metabolic enzymes, we studied upstream players of mir-122 biogenesis in the presence and absence of butyrate in Huh7 cells and mice model.

Silver nanoparticle anchored carbon dots for improved sensing, catalytic and intriguing antimicrobial activity.

Fluorescent carbon dots (NSCDs) with a size of ∼5 nm (λex = 320 nm and λem = 386 nm) have been synthesized under reflux from an alkaline mixture of dopamine and cysteine. The synthesized NSCDs are hybridized with in situ generated silver nanoparticles (AgNPs) obtained by mixing AgNO3 at room temperature. NSCDs enrich the plasmonic bands of AgNPs due to the localized surface plasmon resonance (LSPR) effect.

Fabrication of superhydrophobic copper surface on various substrates for roll-off, self-cleaning, and water/oil separation.

Superhydrophobic surfaces prevent percolation of water droplets and thus render roll-off, self-cleaning, corrosion protection, etc., which find day-to-day and industrial applications. In this work, we developed a facile, cost-effective, and free-standing method for direct fabrication of copper nanoparticles to engender superhydrophobicity for various flat and irregular surfaces such as glass, transparency sheet (plastic), cotton wool, textile, and silicon substrates.

Process optimization of xylanase production using cheap solid substrate by Trichoderma reesei SAF3 and study on the alteration of behavioral properties of enzyme obtained from SSF and SmF.

This study aimed to assess the variability in respect of titer and properties of xylanase from Trichoderma reesei SAF3 under both solid-state and submerged fermentation. SSF was initially optimized with different agro-residues and among them wheat bran was found to be the best substrate that favored maximum xylanase production of 219 U (gws)(-1) at 96 h of incubation. The mycelial stage of the fungi and intracellular accumulation of Ca(++) and Mg(++) induced maximum enzyme synthesis.

Enhanced production and partial characterization of an extracellular polysaccharide from newly isolated Azotobacter sp. SSB81.

A strain was selected by its highest extracellular polysaccharide (EPS) production ability compare to other isolates from the same rhizospheric soil. The selected strain was identified by 16S rDNA sequencing and designated as SSB81. Phylogenetic analysis of the gene sequence showed its close relatedness with Azotobacter vinelandii and Azotobacter salinestris.