A Multichamber Gas System to Examine the Effect of Multiple Oxygen Conditions on Cell Culture.

The classic bone tissue engineering model for bone regeneration combines three elements: scaffolds, biomaterials, and mesenchymal stem cells (MSCs). Incorporation of MSCs and growth factors into a scaffold implanted into the area of bone injury is a proven strategy to achieve successful bone regeneration as demonstrated in the literature. However, a major limitation of using bone grafts or scaffolds is oxygen (O) deprivation in the inner sections of the construct, due to lack of adequate vascularization.